Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224045_u548 ATGGTATGAATTGTATTGTAATGCTAAATCTG(+ACTCAC)GTACCTGAAGGTCTAGGCCTGTGAGTGAATT NGG 10 8 17 8 24 0 100 5 10
    240604224044_o98r GTGAGAATTCACTCACAGGCCTAGACCTTCAG(+ACTCAC)GTACCAGATTTAGCATTACAATACAATTCAT NGG 10 8 17 8 24 0 100 5 10
    240604224043_nwd8 TTTGTAAGCCAGTTGGGGATAAGCTTATATTG(+ACTCAC)GTACCACATGGGAGAAAAAGAACCAGGACCT NGG 10 8 17 8 24 0 100 5 10
    240604224041_zv2m ATTCAAGGTCCTGGTTCTTTTTCTCCCATGTG(+ACTCAC)GTACCAATATAAGCTTATCCCCAACTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240604224040_mtzj CTTGTGAAGCTCAGGAGCTGTGATCATATCAG(+ACTCAC)GTACCAGCATATGCCTTCTCAGTCATGATCC NGG 10 8 17 8 24 0 100 5 10
    240604224039_8ztz AGACAGGATCATGACTGAGAAGGCATATGCTG(+ACTCAC)GTACCTGATATGATCACAGCTCCTGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240604224038_wmh0 TAATATGCAGAATCCTCTACCTGAGAAAAATG(+ACTCAC)GTACCCTGAGAGCAGAAGTGCCTTGCCTGTC NGG 10 8 17 8 24 0 100 5 10
    240604224036_73oz TGGAAGACAGGCAAGGCACTTCTGCTCTCAGG(+ACTCAC)GTACCATTTTTCTCAGGTAGAGGATTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604224035_e2xd ATAACCCCAGAGTTGGGAGATAAAGACAAGAG(+ACTCAC)GATCCATGGGAATTGTAGTTCAACCAGTTTA NGG 10 8 17 8 24 0 100 5 10
    240604224032_tagr TTGGCTAAACTGGTTGAACTACAATTCCCATG(+ACTCAC)GATCCTCTTGTCTTTATCTCCCAACTCTGGG NGG 10 8 17 8 24 0 100 5 10