Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223941_o98y ACAAATCTTACTCATACACTGATTCTTTCCTG(+ACTCAC)GATCCCTGGGAAAGGAGCAGTACACTAGACA NGG 10 8 17 8 24 0 100 5 10
    240604223938_qgfw TTAAATGTCTAGTGTACTGCTCCTTTCCCAGG(+ACTCAC)GATCCAGGAAAGAATCAGTGTATGAGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240604223936_m19n TGGCATTTGACTGGTGGAGATGGATTACATTG(+ACTCAC)GTACCATAAATGATGGAACTCTGCAATGGAA NGG 10 8 17 8 24 0 100 5 10
    240604223935_rdz8 GGCCTTTCCATTGCAGAGTTCCATCATTTATG(+ACTCAC)GTACCAATGTAATCCATCTCCACCAGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240604223932_m5zg AGGGTAAACAAGGGAGACTGCTGCAGCCCCTG(+ACTCAC)GATCCCAAATGCCCATGTCTGAGCCACACGA NGG 10 8 17 8 24 0 100 5 10
    240604223930_9le1 GAGCTTCGTGTGGCTCAGACATGGGCATTTGG(+ACTCAC)GATCCAGGGGCTGCAGCAGTCTCCCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240604223927_ovn2 AGGGTAAACAAGGGAGACTGCTGCAGCCCCTG(+ACTCAC)GATCCCAAATGCCCATGTCTGAGCCACACGA NGG 10 8 17 8 24 0 100 5 10
    240604223925_w2yg GAGCTTCGTGTGGCTCAGACATGGGCATTTGG(+ACTCAC)GATCCAGGGGCTGCAGCAGTCTCCCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240604223924_dms8 CAAAGGCTCCAGTTCCTACAAGTTGGTAGAGG(+ACTCAC)GATCCAGGTGTACTTTCAGTCACTGGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604223922_2kzo TGTAATTCTTCCAGTGACTGAAAGTACACCTG(+ACTCAC)GATCCCTCTACCAACTTGTAGGAACTGGAGC NGG 10 8 17 8 24 0 100 5 10