Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223832_2d7v CCTTCTACCAAGTGGGTAGTCCGTCCTGATAG(+ACTCAC)GTACCTTTACCTGCTGAGCCATCTGAGTAGC NGG 10 8 17 8 24 0 100 5 10
    240604223831_54xk GTGAGGCTACTCAGATGGCTCAGCAGGTAAAG(+ACTCAC)GTACCTATCAGGACGGACTACCCACTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240604223829_tgh7 CAGTGGGTTGCTGCTCTCCAGGGATGTGGCTG(+ACTCAC)GTACCCCAGGTTCTTCCAGGTCATTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240604223827_u90g AGAGGCAGGAGAATGACCTGGAAGAACCTGGG(+ACTCAC)GTACCAGCCACATCCCTGGAGAGCAGCAACC NGG 10 8 17 8 24 0 100 5 10
    240604223826_9r5i GTGGACTGCATCCGACTGAAATAAGCAAAATG(+ACTCAC)GTACCACAGCGCCCGGGTGCACACAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240604223824_5n7s TTCATGGCTGCTGTGTGCACCCGGGCGCTGTG(+ACTCAC)GTACCATTTTGCTTATTTCAGTCGGATGCAG NGG 10 8 17 8 24 0 100 5 10
    240604223822_zwxt GTAAGACTGGGAGAAAGATTTCTTAACCAGTG(+ACTCAC)GTACCTGACTCTTGACCTGGTCATGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240604223821_6rs4 ATTCCCAGGACCATGACCAGGTCAAGAGTCAG(+ACTCAC)GTACCACTGGTTAAGAAATCTTTCTCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240604223819_svjm AGAATTTTCACGGCAGGTACATAAAGGAAGTG(+ACTCAC)GTACCCAGTCTCCAGTCAGGACGTGAGCACC NGG 10 8 17 8 24 0 100 5 10
    240604223817_7ysy AGCCTGGTGCTCACGTCCTGACTGGAGACTGG(+ACTCAC)GTACCACTTCCTTTATGTACCTGCCGTGAAA NGG 10 8 17 8 24 0 100 5 10