Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223903_2bbm GGGAAGAACTGTTAAGTATCGCTAGGGTCAGG(+ACTCAC)GATCCTGACACCTTGGTCAGCTACCAAACAC NGG 10 8 17 8 24 0 100 5 10
    240604223902_f6l0 CAAGAGTGTTTGGTAGCTGACCAAGGTGTCAG(+ACTCAC)GATCCCTGACCCTAGCGATACTTAACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240604223901_2euv GCTCCAGTGCCTACAGCTGCAGGGTGGCATTG(+ACTCAC)GATCCTGTGACTCCCGGTCCTTCCTAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240604223859_4h09 TCCTTTCACTTAGGAAGGACCGGGAGTCACAG(+ACTCAC)GATCCAATGCCACCCTGCAGCTGTAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240604223858_2koq TTTAATTAAAGAGAATTCTTCCACTTCGTCGG(+ACTCAC)GATCCGTCTTCTGCAATCAAAGTGGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240604223856_g5pk CCTCAAGCAGTCCACTTTGATTGCAGAAGACG(+ACTCAC)GATCCCGACGAAGTGGAAGAATTCTCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240604223854_uu3s TGTGCTGTCACCACTCCCCAATGTCCTGGGAG(+ACTCAC)GTACCTCCAGTCAGAGGCAAAGAGGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240604223853_00ee CTCTGTCATGGCCTCTTTGCCTCTGACTGGAG(+ACTCAC)GTACCTCCCAGGACATTGGGGAGTGGTGACA NGG 10 8 17 8 24 0 100 5 10
    240604223851_5qi5 TGTGGGGTGGGCTCTGGAGGCAGGCGACAGTG(+ACTCAC)GTACCAGGCTGCCTACTGAGTGTCTATGGGG NGG 10 8 17 8 24 0 100 5 10
    240604223849_k5hn AGCGCCCCCATAGACACTCAGTAGGCAGCCTG(+ACTCAC)GTACCACTGTCGCCTGCCTCCAGAGCCCACC NGG 10 8 17 8 24 0 100 5 10