Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223920_x3c9 TGTCCGGGTCCCATGTGAGTGACTCTAGGAAG(+ACTCAC)GATCCTAGGATTGTGAAGTGAACAGTAACAC NGG 10 8 17 8 24 0 100 5 10
    240604223918_hf98 GACAAGTGTTACTGTTCACTTCACAATCCTAG(+ACTCAC)GATCCTTCCTAGAGTCACTCACATGGGACCC NGG 10 8 17 8 24 0 100 5 10
    240604223916_hjeg ACACCGAGGAACCCCAGTTAGGAAGATCTGGG(+ACTCAC)GTACCAGAGAATGCTTGCCCTTTCCTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604223915_9bgq GCCAACCTGAAGGAAAGGGCAAGCATTCTCTG(+ACTCAC)GTACCCCAGATCTTCCTAACTGGGGTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240604223913_ne8k CAGTGAGCATCCTGCCTGCCTATGCATTTTGG(+ACTCAC)GTACCTCCCCTGTGACACCCACCCCCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240604223911_jc0e ATACAGCTCTGGGGGTGGGTGTCACAGGGGAG(+ACTCAC)GTACCCAAAATGCATAGGCAGGCAGGATGCT NGG 10 8 17 8 24 0 100 5 10
    240604223909_nz1q CTTGTATACTGCAAGACAGGTCACACACCAAG(+ACTCAC)GATCCTTCAAACACAAACGCTCCTCCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240604223908_bp60 ATCAAGAAGGGGAGGAGCGTTTGTGTTTGAAG(+ACTCAC)GATCCTTGGTGTGTGACCTGTCTTGCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240604223906_86sp TCAAGAGGCAAAGTCTCCACTTTCCTACACAG(+ACTCAC)GATCCATCTTTTGTCTCAGCCAACTCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240604223905_kbo7 CCTCCATTGAGAGTTGGCTGAGACAAAAGATG(+ACTCAC)GATCCTGTGTAGGAAAGTGGAGACTTTGCCT NGG 10 8 17 8 24 0 100 5 10