Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224015_kxpc CGTGACATGGGTTAGGCTCGAACTGCACAGTG(+ACTCAC)GTACCAATGTGGTCTGGCCTTGAACAACTCA NGG 10 8 17 8 24 0 100 5 10
    240604224013_lcg0 CTTAGTGAGTTGTTCAAGGCCAGACCACATTG(+ACTCAC)GTACCACTGTGCAGTTCGAGCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10
    240604224012_m01h AAAAAAAATCACACGTGGAAGAAAAGAAGTCG(+ACTCAC)GTACCAGTACAAAAAGACTGCAGGGCCTACT NGG 10 8 17 8 24 0 100 5 10
    240604224011_in2q CTGACAGTAGGCCCTGCAGTCTTTTTGTACTG(+ACTCAC)GTACCGACTTCTTTTCTTCCACGTGTGATTT NGG 10 8 17 8 24 0 100 5 10
    240604224010_f7ob GTCCCTCCACGGTTTCACTCATGAGGGGCTCG(+ACTCAC)GATCCTTCTTGTCTCAATGACGTGCATTTCT NGG 10 8 17 8 24 0 100 5 10
    240604224008_t02n TTCAGAGAAATGCACGTCATTGAGACAAGAAG(+ACTCAC)GATCCGAGCCCCTCATGAGTGAAACCGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604224007_4koh ATAAAGCAAACTCTGCTATTTGAATCTTGTTG(+ACTCAC)GTACCTGTTGGCCAGTGCCATACCCTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604224006_rcn4 GACTGTCAGGAGGGTATGGCACTGGCCAACAG(+ACTCAC)GTACCAACAAGATTCAAATAGCAGAGTTTGC NGG 10 8 17 8 24 0 100 5 10
    240604224003_uycs CTCCTATGCTTCCAACTCACCCCACCACCCTG(+ACTCAC)GATCCTCTCTAAGCACCCATCTCTTGACTCA NGG 10 8 17 8 24 0 100 5 10
    240604224002_tjk7 GAGTGTGAGTCAAGAGATGGGTGCTTAGAGAG(+ACTCAC)GATCCAGGGTGGTGGGGTGAGTTGGAAGCAT NGG 10 8 17 8 24 0 100 5 10