Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224118_oz0k ACTTGCTTTTTTTTTTTTTTAATTTGATGCTG(+ACTCAC)GATCCAGAATAGGCCAGTGAAGATCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240604224117_v7vt ACCACTGCTCTGATCTTCACTGGCCTATTCTG(+ACTCAC)GATCCAGCATCAAATTAAAAAAAAAAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240604224115_m0j3 TAACTTGAACTTGCAGGTCACCTCTGAACAGG(+ACTCAC)GTACCCTTGTCCCCACCCCAGGCCCTGTTAC NGG 10 8 17 8 24 0 100 5 10
    240604224113_zbgm ACAGGGTAACAGGGCCTGGGGTGGGGACAAGG(+ACTCAC)GTACCCTGTTCAGAGGTGACCTGCAAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604224110_0ztk TCTCCTGTGTAGACTAGCTGGAGGTCCCTGGG(+ACTCAC)GATCCCCAGGGAAAGGACGCTCTGCCGCGCA NGG 10 8 17 8 24 0 100 5 10
    240604224107_s8i4 GAGATTGCGCGGCAGAGCGTCCTTTCCCTGGG(+ACTCAC)GATCCCCAGGGACCTCCAGCTAGTCTACACA NGG 10 8 17 8 24 0 100 5 10
    240604224105_c7df CTTTGTCCTCGTCGAGCTGCTCTGAGCTGTGG(+ACTCAC)GATCCTGTGAACAACTGTCGTCTTTCCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604224104_etv7 TGAAGAGAAGGAAAGACGACAGTTGTTCACAG(+ACTCAC)GATCCCACAGCTCAGAGCAGCTCGACGAGGA NGG 10 8 17 8 24 0 100 5 10
    240604224103_imea CCAAAATGCCCGTCCTATAGAGTGTCTGATTG(+ACTCAC)GATCCTCTGTTGCGTATTCTGATGGTGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604224101_n2yd ACCAGCAGGCACCATCAGAATACGCAACAGAG(+ACTCAC)GATCCAATCAGACACTCTATAGGACGGGCAT NGG 10 8 17 8 24 0 100 5 10