Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224208_jz37 AAGCCCCTCTTGTTCCTCCATCACTAAAAGAG(+ACTCAC)GATCCCTGAAATCAGACCAAAGCCACACATC NGG 10 8 17 8 24 0 100 5 10
    240604224207_y1v8 TATTTGATGTGTGGCTTTGGTCTGATTTCAGG(+ACTCAC)GATCCTCTTTTAGTGATGGAGGAACAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604224204_wthv CACAGAGAAGCCTGGCAGAATGGAACCCAGTG(+ACTCAC)GTACCTGCCCCACAAGAATTCTAGGACATTC NGG 10 8 17 8 24 0 100 5 10
    240604224203_2hgg GGTGTGAATGTCCTAGAATTCTTGTGGGGCAG(+ACTCAC)GTACCACTGGGTTCCATTCTGCCAGGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604224202_v5j5 GTTGGCCTTCTCACTTTGATAGTTTAACTCAG(+ACTCAC)GTACCCAGTCTTCACTCCATCCCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240604224200_2sgt GAACAAGCACCTGGGGATGGAGTGAAGACTGG(+ACTCAC)GTACCTGAGTTAAACTATCAAAGTGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240604224158_cgy2 AACTTCAAAGCTTTATTCTGTCTGCCTGAGAG(+ACTCAC)GATCCATGGAAGATGATTCTCCCAAAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240604224156_kiyf TTTTCTGCTTTTTGGGAGAATCATCTTCCATG(+ACTCAC)GATCCTCTCAGGCAGACAGAATAAAGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604224155_vsk0 GAAGGCTTTCTACTCCTTAACAGGTACAGTTG(+ACTCAC)GATCCATCGCTGATGGTGTTTCTTTAGCCCC NGG 10 8 17 8 24 0 100 5 10
    240604224153_0u9t GGGATGGGGCTAAAGAAACACCATCAGCGATG(+ACTCAC)GATCCAACTGTACCTGTTAAGGAGTAGAAAG NGG 10 8 17 8 24 0 100 5 10