Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223959_9r7f GACTCTTGTCAAGAGGACCCGCACGAAGCCCG(+ACTCAC)GATCCACAGCGAGCTTTTTGGAAGGGAATAA NGG 10 8 17 8 24 0 100 5 10
    240604223958_su7k AACTGTTATTCCCTTCCAAAAAGCTCGCTGTG(+ACTCAC)GATCCGGGCTTCGTGCGGGTCCTCTTGACAA NGG 10 8 17 8 24 0 100 5 10
    240604223955_g1lk GCTCACAACCATCTGTATCTCCAGTCCCAGGG(+ACTCAC)GATCCAGCACCCTCTTCTGGTGTCCTCAGAC NGG 10 8 17 8 24 0 100 5 10
    240604223954_ttvi CTGGTGTCTGAGGACACCAGAAGAGGGTGCTG(+ACTCAC)GATCCCCTGGGACTGGAGATACAGATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240604223952_mauh GAAGCTTAGTGAGGCGGAGGCTGCCAGTGTTG(+ACTCAC)GTACCTGTGGATTTTCCAATAGTGAGGGTTA NGG 10 8 17 8 24 0 100 5 10
    240604223950_skin AGAGCTAACCCTCACTATTGGAAAATCCACAG(+ACTCAC)GTACCAACACTGGCAGCCTCCGCCTCACTAA NGG 10 8 17 8 24 0 100 5 10
    240604223948_e83v GGTCACAGTGAAGTCCCTTGCAGCCCCCAGTG(+ACTCAC)GTACCTTACACCAAGGACTGTGCTCTCAATC NGG 10 8 17 8 24 0 100 5 10
    240604223946_0jmr ACAGTGATTGAGAGCACAGTCCTTGGTGTAAG(+ACTCAC)GTACCACTGGGGGCTGCAAGGGACTTCACTG NGG 10 8 17 8 24 0 100 5 10
    240604223945_2z0h GAAAGTTGTTTTCTTACTCTGTCCTTGGCAGG(+ACTCAC)GTACCTCTTACATTTCCCAGATACGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240604223944_kuuv GCTAGTCAAACCGTATCTGGGAAATGTAAGAG(+ACTCAC)GTACCCTGCCAAGGACAGAGTAAGAAAACAA NGG 10 8 17 8 24 0 100 5 10