Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223739_bdq0 TGCCCCAGGCTCCAGAGCAAGTAGGACGTCAG(+ACTCAC)GTACCTGCTCTCTTGCCTGGCTCTAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240604223737_ezp8 AAATTAAAATCTAGAGCCAGGCAAGAGAGCAG(+ACTCAC)GTACCTGACGTCCTACTTGCTCTGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240604223736_9za9 GCCAAGTGGGACGCATTGACTGGTCACACGTG(+ACTCAC)GTACCTCTGGCCTTTCTTTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240604223734_i378 AAATAAATAAATAAATAAAAGAAAGGCCAGAG(+ACTCAC)GTACCACGTGTGACCAGTCAATGCGTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240604223733_yjq6 CCCTGGGCCAAACATATTCAAACCACCATAAG(+ACTCAC)GTACCCAGAGAACAACCCCAGAACTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240604223731_fijb TCTCATAGTGCAGTTCTGGGGTTGTTCTCTGG(+ACTCAC)GTACCTTATGGTGGTTTGAATATGTTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240604223730_d062 CTTAGTCCTGAGACTGCCAGACAATCAAGCAG(+ACTCAC)GTACCCCACCAGGAGGCCAGCTCTGGACCTC NGG 10 8 17 8 24 0 100 5 10
    240604223728_q28j TGGCAGAGGTCCAGAGCTGGCCTCCTGGTGGG(+ACTCAC)GTACCTGCTTGATTGTCTGGCAGTCTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240604223726_m93v CTTAGGGTTTTCTTTGCTCCTTTGTAAGGTGG(+ACTCAC)GATCCTCTTGCCAGTTTTCTCCAGCTGTGCC NGG 10 8 17 8 24 0 100 5 10
    240604223725_c69a GGTGTGGCACAGCTGGAGAAAACTGGCAAGAG(+ACTCAC)GATCCCACCTTACAAAGGAGCAAAGAAAACC NGG 10 8 17 8 24 0 100 5 10