Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223650_edf1 TTCCAAGTGAAGTCTGCTAAGCAGACCTCAGG(+ACTCAC)GTACCTGGCAGAGCCTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604223648_a6bn CATTCACACCCAGTTCACACAGGCTCTGCCAG(+ACTCAC)GTACCCTGAGGTCTGCTTAGCAGACTTCACT NGG 10 8 17 8 24 0 100 5 10
    240604223647_ht5o GTGCAAATTTCCAGAAAATAATGGAGATACAG(+ACTCAC)GTACCATAGGACAGTGAAGCAAGAATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240604223645_80pq CTGGAGAGACATTCTTGCTTCACTGTCCTATG(+ACTCAC)GTACCTGTATCTCCATTATTTTCTGGAAATT NGG 10 8 17 8 24 0 100 5 10
    240604223643_37x1 GTTTTAGTTTACAAAAGAAAAATCAATGATTG(+ACTCAC)GTACCTTTTTTTAACACTCTCAGATTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240604223642_gr04 CATATTCAGGAATCTGAGAGTGTTAAAAAAAG(+ACTCAC)GTACCAATCATTGATTTTTCTTTTGTAAACT NGG 10 8 17 8 24 0 100 5 10
    240604223640_vyuk TGAAGACAATAAATATTCCTTTGAGACCCGTG(+ACTCAC)GATCCTTCTGCGGACCCTAATGACACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240604223638_yw6m GCTCCACTTTGTGTCATTAGGGTCCGCAGAAG(+ACTCAC)GATCCACGGGTCTCAAAGGAATATTTATTGT NGG 10 8 17 8 24 0 100 5 10
    240604223637_a6u2 ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+ACTCAC)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240604223636_8b2z GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+ACTCAC)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10