Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223633_xtdq TCCTTTGCCTTATCTCATGGGGATGGAGGAGG(+ACTCAC)GTACCCAGGTCTCTGTTGCTTTGACTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240604223631_t981 TTATTGAGACAGTCAAAGCAACAGAGACCTGG(+ACTCAC)GTACCCTCCTCCATCCCCATGAGATAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604223630_jyy0 AGTACCTGAGGGTCTGTAGGTTGCACACTTTG(+ACTCAC)GTACCAGGTAACTTTTTTTTCTTTATAAGAA NGG 10 8 17 8 24 0 100 5 10
    240604223628_ap0k ACTCTTTCTTATAAAGAAAAAAAAGTTACCTG(+ACTCAC)GTACCAAAGTGTGCAACCTACAGACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604223626_3ytk TCTTCCCACTGTGTAGTCCTGGGTCTTGTTAG(+ACTCAC)GATCCTGGCTGGGGCCTCTGGGAGCAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240604223624_vr6i GCTCGACTTTTGCTCCCAGAGGCCCCAGCCAG(+ACTCAC)GATCCTAACAAGACCCAGGACTACACAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604223622_gp6m AGCAGTCTCCCAGTGCATGTTTGCATAGCTCG(+ACTCAC)GTACCAGAGCGGTTTGTAATTGAGCCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240604223621_emmg ACTGCAAACAGGCTCAATTACAAACCGCTCTG(+ACTCAC)GTACCGAGCTATGCAAACATGCACTGGGAGA NGG 10 8 17 8 24 0 100 5 10
    240604223620_462y GCTCTTTAAGGCAGAGGAGACAGACATGTGTG(+ACTCAC)GATCCGGGGCTGTTGGCTCATCTCACGTGGT NGG 10 8 17 8 24 0 100 5 10
    240604223617_7n2j CTGAAACCACGTGAGATGAGCCAACAGCCCCG(+ACTCAC)GATCCACACATGTCTGTCTCCTCTGCCTTAA NGG 10 8 17 8 24 0 100 5 10