Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223544_o19k TAGTAAAGCTGTCCTCCCTGCCTCTCTCACTG(+ACTCAC)GATCCGGATTGGCAACATTCCTGACTGACCC NGG 10 8 17 8 24 0 100 5 10
    240604223542_dbjj TCTGAGGGTCAGTCAGGAATGTTGCCAATCCG(+ACTCAC)GATCCAGTGAGAGAGGCAGGGAGGACAGCTT NGG 10 8 17 8 24 0 100 5 10
    240604223541_mh83 CACAGCAGAACATGAATGTTCTGTATGTTTTG(+ACTCAC)GTACCCAAATCTCTAGTACACAGTACACATC NGG 10 8 17 8 24 0 100 5 10
    240604223539_m5lv GGGCAGATGTGTACTGTGTACTAGAGATTTGG(+ACTCAC)GTACCAAAACATACAGAACATTCATGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240604223538_jzvv ATTGCCATGTAGGTGCTGGGAATTGAATTCAG(+ACTCAC)GTACCCTAGAAGAACAGCCAGTGTTCTTAGC NGG 10 8 17 8 24 0 100 5 10
    240604223536_we1p CGGGGGCTAAGAACACTGGCTGTTCTTCTAGG(+ACTCAC)GTACCTGAATTCAATTCCCAGCACCTACATG NGG 10 8 17 8 24 0 100 5 10
    240604223534_ykgw TCTTCTCAATATCCGTCTACACATGGCATTGG(+ACTCAC)GTACCATCCCTCTCAGTGTCTATTGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240604223532_udk5 CTCTGAGAGAACAATAGACACTGAGAGGGATG(+ACTCAC)GTACCCAATGCCATGTGTAGACGGATATTGA NGG 10 8 17 8 24 0 100 5 10
    240604223531_ezyt CTGGTTTACCTATGGAGGGAAGTGCTAGAATG(+ACTCAC)GATCCTGGACTAAACTCCCCCTGATCACCAC NGG 10 8 17 8 24 0 100 5 10
    240604223529_shoy ACAGTGTGGTGATCAGGGGGAGTTTAGTCCAG(+ACTCAC)GATCCATTCTAGCACTTCCCTCCATAGGTAA NGG 10 8 17 8 24 0 100 5 10