Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223513_ihwf AGCACCCTTGTGTCTCTGGCTTCCTGTCACTG(+ACTCAC)GATCCAGTACCCCTCCATGCTTGGGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240604223512_ojt6 TAAAACAAGGACCCAAGCATGGAGGGGTACTG(+ACTCAC)GATCCAGTGACAGGAAGCCAGAGACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240604223510_o88g CTTAATATTTTCAAACTAATAGTTTATTATAG(+ACTCAC)GTACCATAAATCTTTAAATAAACACTATTTT NGG 10 8 17 8 24 0 100 5 10
    240604223509_lbes AAAATAAAATAGTGTTTATTTAAAGATTTATG(+ACTCAC)GTACCTATAATAAACTATTAGTTTGAAAATA NGG 10 8 17 8 24 0 100 5 10
    240604223508_9swk TGGAATGTCTTGGAGGCCAGAGGAGGGTGTTG(+ACTCAC)GATCCCTTAGGACTGGAGTTAAAGACGAGAG NGG 10 8 17 8 24 0 100 5 10
    240604223506_5bia CTCACCTCTCGTCTTTAACTCCAGTCCTAAGG(+ACTCAC)GATCCAACACCCTCCTCTGGCCTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240604223505_6b1o ATCTAGGCTCTGGTCCTCATCTGGGGTCAAAG(+ACTCAC)GATCCCTTAGGAGGGGTCAGTTTTGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240604223503_w94t ACAATCAGAAGCAAAACTGACCCCTCCTAAGG(+ACTCAC)GATCCTTTGACCCCAGATGAGGACCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604223501_ihgv TTTAAAGAACCACAGTGTGCAAATGTTTCAGG(+ACTCAC)GATCCTAAGCAATGCCCCAACACATGAACAG NGG 10 8 17 8 24 0 100 5 10
    240604223459_du1z CCAGCCTGTTCATGTGTTGGGGCATTGCTTAG(+ACTCAC)GATCCCTGAAACATTTGCACACTGTGGTTCT NGG 10 8 17 8 24 0 100 5 10