Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223405_8u39 GCCAGTTCCTAGCTGATCACAGCTTTCCTTAG(+ACTCAC)GATCCAGGAAGGTCTGTGTTTTCTGAGAATA NGG 10 8 17 8 24 0 100 5 10
    240604223402_6y5d AAGACTATTCTCAGAAAACACAGACCTTCCTG(+ACTCAC)GATCCTAAGGAAAGCTGTGATCAGCTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604223400_umzh TTTAAAAATGAAGCCCAGCTGGGTGTGGTGGG(+ACTCAC)GTACCCTTCTAATCCCAGTACTTGGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604223358_x7xq TTCTCTGCCTCCCAAGTACTGGGATTAGAAGG(+ACTCAC)GTACCCCACCACACCCAGCTGGGCTTCATTT NGG 10 8 17 8 24 0 100 5 10
    240604223355_15t8 GCTGCTTCAACCAGACTGAGCTTGGTGCCTGG(+ACTCAC)GTACCCAGTGATGAGCCAAGTAAGGTAGGCA NGG 10 8 17 8 24 0 100 5 10
    240604223353_3pja GCACTTGCCTACCTTACTTGGCTCATCACTGG(+ACTCAC)GTACCCAGGCACCAAGCTCAGTCTGGTTGAA NGG 10 8 17 8 24 0 100 5 10
    240604223352_i4id CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+ACTCAC)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240604223350_1q0f GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+ACTCAC)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240604223347_36us CAAAGTGCTCAGAGTTCCAGCCCTGGGCCCCG(+ACTCAC)GTACCCATATCCATACCACTCCCTGGCTCGG NGG 10 8 17 8 24 0 100 5 10
    240604223346_how8 GTAAACCGAGCCAGGGAGTGGTATGGATATGG(+ACTCAC)GTACCGGGGCCCAGGGCTGGAACTCTGAGCA NGG 10 8 17 8 24 0 100 5 10