Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223312_yiqg GTTCTGCCAAGACCGGCAGGGTAAACAAACAG(+ACTCAC)GTACCACTCTCTCCCGGGAACCCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240604223311_4qej ACAACAGAAGAAAGGGTTCCCGGGAGAGAGTG(+ACTCAC)GTACCTGTTTGTTTACCCTGCCGGTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240604223309_2k9r CAGGAAAGGTTCCGTTTATAAACAAGAAACTG(+ACTCAC)GTACCAGAAAATTGCAGAGAAGCACCAACGT NGG 10 8 17 8 24 0 100 5 10
    240604223308_z7cu GGAGCACGTTGGTGCTTCTCTGCAATTTTCTG(+ACTCAC)GTACCAGTTTCTTGTTTATAAACGGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240604223306_vilr AAATAGGGAAAGAAGTCAGTGGCTGGTAGAGG(+ACTCAC)GTACCACTCCCCAGATGCTGTGTAGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240604223305_lx9t CAGGGCCTCAACTACACAGCATCTGGGGAGTG(+ACTCAC)GTACCCTCTACCAGCCACTGACTTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604223303_iul8 CTAAGCAGCCAATGTCGCTTCTGGGGCTCCTG(+ACTCAC)GATCCTCTAGGCGGAGTCCTTTATGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240604223301_re0m GGGGGGGACACCATAAAGGACTCCGCCTAGAG(+ACTCAC)GATCCAGGAGCCCCAGAAGCGACATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240604223300_3ssz CTTTCCTTCCACACTGTCTTGTATCATGGCTG(+ACTCAC)GATCCAGTGTATTTCCAGCTAATTGTTAATG NGG 10 8 17 8 24 0 100 5 10
    240604223258_zd32 CATCACATTAACAATTAGCTGGAAATACACTG(+ACTCAC)GATCCAGCCATGATACAAGACAGTGTGGAAG NGG 10 8 17 8 24 0 100 5 10