Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223344_ur9g GGAGACAAGATATGACAGGAAGAAACTTTTAG(+ACTCAC)GTACCTGGGCAGAAAGAGCTGTTGTTTGAAA NGG 10 8 17 8 24 0 100 5 10
    240604223341_fl98 GGCCCTTTCAAACAACAGCTCTTTCTGCCCAG(+ACTCAC)GTACCTAAAAGTTTCTTCCTGTCATATCTTG NGG 10 8 17 8 24 0 100 5 10
    240604223340_quqn GAATGGCAAGAGGATCAAGGTCAGAATGTCTG(+ACTCAC)GTACCGTCTGTGGAATCTAAGATAGGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240604223339_u3vq GGCTGGGAGCCCTATCTTAGATTCCACAGACG(+ACTCAC)GTACCAGACATTCTGACCTTGATCCTCTTGC NGG 10 8 17 8 24 0 100 5 10
    240604223335_h6wq GCAGGGCTGTTGGGCTCTGAGTGGAGCCCGGG(+ACTCAC)GTACCCACAGGGTGGAAGGAGAGGGAGCGGG NGG 10 8 17 8 24 0 100 5 10
    240604223333_1zmf CCACCCCCGCTCCCTCTCCTTCCACCCTGTGG(+ACTCAC)GTACCCCGGGCTCCACTCAGAGCCCAACAGC NGG 10 8 17 8 24 0 100 5 10
    240604223332_r2wz GGCCCGACACGATGTTCTCTGCACTCCAAATG(+ACTCAC)GTACCTAAGTCATGCTGTTCAAGAACGAGGC NGG 10 8 17 8 24 0 100 5 10
    240604223331_upn2 AAAACGCCTCGTTCTTGAACAGCATGACTTAG(+ACTCAC)GTACCATTTGGAGTGCAGAGAACATCGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604223329_431w CCACCCTATGTCTATTAAGCCTGAGGGCAGGG(+ACTCAC)GATCCTGAGCACACTTTGGGCAGAAATAAGT NGG 10 8 17 8 24 0 100 5 10
    240604223327_vcxj TCGTGACTTATTTCTGCCCAAAGTGTGCTCAG(+ACTCAC)GATCCCCTGCCCTCAGGCTTAATAGACATAG NGG 10 8 17 8 24 0 100 5 10