Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223206_2hi9 TTGTTTCCTCCCAAATACTCCCTGAACGGGAG(+ACTCAC)GATCCCCTTCTGCTCCATCAGGACTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240604223204_zksl GGCTTCAGTGGAGTCCTGATGGAGCAGAAGGG(+ACTCAC)GATCCTCCCGTTCAGGGAGTATTTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240604223203_xhi2 CCCACCACGCTCCCTAGCTGGGAGAGAAACAG(+ACTCAC)GATCCACAAGAGGAGTGGGAAAGCCGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604223202_w31n GTGTCAAAAACGGCTTTCCCACTCCTCTTGTG(+ACTCAC)GATCCTGTTTCTCTCCCAGCTAGGGAGCGTG NGG 10 8 17 8 24 0 100 5 10
    240604223200_q7o1 TAGGCTATATACCATGTTTGCAGTCAGCCTGG(+ACTCAC)GATCCATGAAACCCTGTCTCAAGAAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240604223159_nc3h TATTTTTGCTTTTCTTGAGACAGGGTTTCATG(+ACTCAC)GATCCCAGGCTGACTGCAAACATGGTATATA NGG 10 8 17 8 24 0 100 5 10
    240604223158_pc4b CTAGGGGCTGTGGAGGCTGGAGGAGAGGATCG(+ACTCAC)GATCCCTTAAATACCCATGTGGATGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240604223156_hwiy TCAGTTCCCAGCATCCACATGGGTATTTAAGG(+ACTCAC)GATCCGATCCTCTCCTCCAGCCTCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240604223154_arr4 AAGTCCTTGCTCTTAGCCAGACTAGAGATTAG(+ACTCAC)GATCCCACATGTTTATGCTGTTTGTTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240604223153_rd14 CTATCCCAAAAACAAACAGCATAAACATGTGG(+ACTCAC)GATCCTAATCTCTAGTCTGGCTAAGAGCAAG NGG 10 8 17 8 24 0 100 5 10