Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223115_nwv0 CTAATATACCTCTGTTCCCAGGAGCTACTGAG(+ACTCAC)GTACCCAGTGTGGTGGGGAAAAGACGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240604223114_33du CTTAATTCACCGTCTTTTCCCCACCACACTGG(+ACTCAC)GTACCTCAGTAGCTCCTGGGAACAGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240604223112_2tdf TTAAACATGAAGCCTTCCCTGTGCTGGGCATG(+ACTCAC)GATCCAGGCCAATGTTGGTGTCCTACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604223111_b3vn TGGGCCCCAGGTAGGACACCAACATTGGCCTG(+ACTCAC)GATCCATGCCCAGCACAGGGAAGGCTTCATG NGG 10 8 17 8 24 0 100 5 10
    240604223109_871o CTATATCAAGCAGAACACAGGTCATCAGCTTG(+ACTCAC)GATCCATGATTTGACTTCAAGTCATTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604223107_3usb CAGTGCAAAGAATGACTTGAAGTCAAATCATG(+ACTCAC)GATCCAAGCTGATGACCTGTGTTCTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240604223104_t5ab GCTGGGGGTACTTGCCATGAAAATTCCCTTGG(+ACTCAC)GATCCTGTGACCCTCTGGGGACAGAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240604223103_ip9q ATGTGTGTGCTTCTGTCCCCAGAGGGTCACAG(+ACTCAC)GATCCCAAGGGAATTTTCATGGCAAGTACCC NGG 10 8 17 8 24 0 100 5 10
    240604223102_4gjt AATCTTGCGGAGTTCCAGAACCATACTGTGAG(+ACTCAC)GTACCACAGGGTCCTGTGGCCCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10
    240604223100_59q5 AGAACAAAACTACTGGGCCACAGGACCCTGTG(+ACTCAC)GTACCTCACAGTATGGTTCTGGAACTCCGCA NGG 10 8 17 8 24 0 100 5 10