Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223044_bewp TACTCTATTACCTGCAGGAAGAAACCCTAAAG(+ACTCAC)GTACCCAAAGAAATAAAATTTGGCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240604223042_wojd AAACATCCACTTGCCAAATTTTATTTCTTTGG(+ACTCAC)GTACCTTTAGGGTTTCTTCCTGCAGGTAATA NGG 10 8 17 8 24 0 100 5 10
    240604223041_5if1 GGTCTTGAACTCAGTATGTAGTCTACATTTTG(+ACTCAC)GATCCTCCTGCCCCCACCTCCTAAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240604223040_9ibe TAATGTCAACACTTAGGAGGTGGGGGCAGGAG(+ACTCAC)GATCCAAAATGTAGACTACATACTGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240604223038_xcn5 CATGAATGTTTTGGACATCAAGCATCCTAATG(+ACTCAC)GTACCTCCTTTGTACAATGCCAAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240604223037_diqc ACCGAGGCTCATTTTGGCATTGTACAAAGGAG(+ACTCAC)GTACCATTAGGATGCTTGATGTCCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240604223036_4fa2 CTGGAGTTGGTCATTTTTGTACACCCACAGTG(+ACTCAC)GTACCTTTTACAGTCAGGTTTGGATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240604223035_9mzb AGCTGCAAAGTATCCAAACCTGACTGTAAAAG(+ACTCAC)GTACCACTGTGGGTGTACAAAAATGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240604223033_dm5k CCCCCAGTTCTCTGGCTGAGGCAACATTCCTG(+ACTCAC)GTACCAGTGCCTATCACTTTGTTTCAGGACT NGG 10 8 17 8 24 0 100 5 10
    240604223031_antc TAGGCAGTCCTGAAACAAAGTGATAGGCACTG(+ACTCAC)GTACCAGGAATGTTGCCTCAGCCAGAGAACT NGG 10 8 17 8 24 0 100 5 10