Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223030_gjk5 GTGTGTGTGTGTGTGTGTGTGTGTACTCTAGG(+ACTCAC)GTACCTCAGTATGTCCTGAAAGTTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240604223028_fwki AAAACTCTGTTGAACTTTCAGGACATACTGAG(+ACTCAC)GTACCCTAGAGTACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240604223027_rswp GAAAAGCACCATGGGAAATCCCTTGGCTTCTG(+ACTCAC)GTACCAGCATGGTAATTGCAAGGGAAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240604223026_q64k TCTGAATTTCTTCCCTTGCAATTACCATGCTG(+ACTCAC)GTACCAGAAGCCAAGGGATTTCCCATGGTGC NGG 10 8 17 8 24 0 100 5 10
    240604223024_6zwc AGAACTTGGGTCTGCTTTCTACAGTCATTATG(+ACTCAC)GTACCAAAACCATATGATTCTTTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240604223023_f5ca GGATAAACTGTACAAAGAATCATATGGTTTTG(+ACTCAC)GTACCATAATGACTGTAGAAAGCAGACCCAA NGG 10 8 17 8 24 0 100 5 10
    240604223022_ojod AGCTTGTTTCTGAGAAAACCAAGTTCTAAGTG(+ACTCAC)GTACCCAAAGGTGTTGCCAGTCCTTCCTATG NGG 10 8 17 8 24 0 100 5 10
    240604223020_4qwn GGATGCATAGGAAGGACTGGCAACACCTTTGG(+ACTCAC)GTACCACTTAGAACTTGGTTTTCTCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240604223019_porj CTCCATGGTTAAGAACACTAGTTTTTGCTGAG(+ACTCAC)GATCCCAGTTCAATTCCCAGTACCCACATGG NGG 10 8 17 8 24 0 100 5 10
    240604223017_upag AGCCACCATGTGGGTACTGGGAATTGAACTGG(+ACTCAC)GATCCTCAGCAAAAACTAGTGTTCTTAACCA NGG 10 8 17 8 24 0 100 5 10