Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222928_e9km TGATGTAGAGTAGTTTATTCTGGTGTTGTGTG(+ACTCAC)GATCCACTCAGTCCACAATCTAAAGACAGCA NGG 10 8 17 8 24 0 100 5 10
    240604222927_03pe CTCAATGCTGTCTTTAGATTGTGGACTGAGTG(+ACTCAC)GATCCACACAACACCAGAATAAACTACTCTA NGG 10 8 17 8 24 0 100 5 10
    240604222925_m8ym GCCTACAACATAACTAACTGACCCTCTTAGAG(+ACTCAC)GATCCCAGTAACACCACATATAAAGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604222924_onal AACCGTCTTTTCTTTATATGTGGTGTTACTGG(+ACTCAC)GATCCTCTAAGAGGGTCAGTTAGTTATGTTG NGG 10 8 17 8 24 0 100 5 10
    240604222922_543n TGGGAGGATCATTAGTTCAAGGCCAAACTAGG(+ACTCAC)GTACCTTGTGATAACCTTTCATAAATATAAG NGG 10 8 17 8 24 0 100 5 10
    240604222921_ctpy AATGTCTTATATTTATGAAAGGTTATCACAAG(+ACTCAC)GTACCCTAGTTTGGCCTTGAACTAATGATCC NGG 10 8 17 8 24 0 100 5 10
    240604222920_x49a TCAGGCCCAGCCAAAGCTACCAGAGCTCTAAG(+ACTCAC)GTACCTGGGGACCAATGGAATTTACACCTAT NGG 10 8 17 8 24 0 100 5 10
    240604222916_gefm TTACCATAGGTGTAAATTCCATTGGTCCCCAG(+ACTCAC)GTACCTTAGAGCTCTGGTAGCTTTGGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240604222915_6lji GAGAAACCAAAATGCTGGTGACCCTTAGAGAG(+ACTCAC)GATCCAGTCACCTTCCTTTAAGTCCTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240604222914_d678 ACTGTGGAGAAGGACTTAAAGGAAGGTGACTG(+ACTCAC)GATCCTCTCTAAGGGTCACCAGCATTTTGGT NGG 10 8 17 8 24 0 100 5 10