Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222840_dxap CGTCATCCTAAACTCACCATCCTTAGAAACGG(+ACTCAC)GATCCTCTCTAGAATATTGTCGCCGGTGACC NGG 10 8 17 8 24 0 100 5 10
    240604222838_ty7l CCTGTGGTCACCGGCGACAATATTCTAGAGAG(+ACTCAC)GATCCCGTTTCTAAGGATGGTGAGTTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240604222837_n9s2 TTACTTTGATGGCTTCTCCAGGTTTTTGTCAG(+ACTCAC)GTACCACATGTAGAATTACATTTTTCTTAAA NGG 10 8 17 8 24 0 100 5 10
    240604222836_st3r AATCTTTTAAGAAAAATGTAATTCTACATGTG(+ACTCAC)GTACCTGACAAAAACCTGGAGAAGCCATCAA NGG 10 8 17 8 24 0 100 5 10
    240604222835_obcd CCTCCATCCGGAGGAAGCAACAGCTGAATCAG(+ACTCAC)GATCCGCAGCGTAAGGGGTCAGCTAATCAGT NGG 10 8 17 8 24 0 100 5 10
    240604222833_o0vm CTCCCACTGATTAGCTGACCCCTTACGCTGCG(+ACTCAC)GATCCTGATTCAGCTGTTGCTTCCTCCGGAT NGG 10 8 17 8 24 0 100 5 10
    240604222832_xrw1 AGCTAGACATTTATTGATAATACATTACAGTG(+ACTCAC)GATCCAAAATCTAGCATTATTTTAGTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240604222831_jdtw CCTGTTCACCACTAAAATAATGCTAGATTTTG(+ACTCAC)GATCCACTGTAATGTATTATCAATAAATGTC NGG 10 8 17 8 24 0 100 5 10
    240604222829_g2a9 ATGTGCCCATCAGGGGGCCGTCTTAACCTTTG(+ACTCAC)GTACCATCTTCTTCAAAGCTGCATTAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240604222828_u6lq TGCTCTCTTTTAATGCAGCTTTGAAGAAGATG(+ACTCAC)GTACCAAAGGTTAAGACGGCCCCCTGATGGG NGG 10 8 17 8 24 0 100 5 10