Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222741_j0wl CTTCATACAGCAGTCATCTGCTCGTATGTTTG(+ACTCAC)GATCCCCTTTTAAACATTCAACCCTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240604222739_40u8 GATGGACCTCCAGGGTTGAATGTTTAAAAGGG(+ACTCAC)GATCCAAACATACGAGCAGATGACTGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240604222737_rx13 ACGATTCCCATTCTCTGTCTGTCTCGTATATG(+ACTCAC)GTACCATCTTCCAGTCACAGATCCTGACTCT NGG 10 8 17 8 24 0 100 5 10
    240604222735_tar8 AGATGAGAGTCAGGATCTGTGACTGGAAGATG(+ACTCAC)GTACCATATACGAGACAGACAGAGAATGGGA NGG 10 8 17 8 24 0 100 5 10
    240604222734_7s7m TCTTCTGAAGTGTATGCTAAGTGTGTGTGGCG(+ACTCAC)GTACCTTCAGAGTCCAGAGGAAGGTATCAGA NGG 10 8 17 8 24 0 100 5 10
    240604222733_od1o CAGGATCTGATACCTTCCTCTGGACTCTGAAG(+ACTCAC)GTACCGCCACACACACTTAGCATACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240604222731_ib2l CATACACTATTCCCGAGTTAGTCTGGTGTATG(+ACTCAC)GATCCACGTGCTCTCTTTTAGTATTATCTTA NGG 10 8 17 8 24 0 100 5 10
    240604222730_yvoe TATAATAAGATAATACTAAAAGAGAGCACGTG(+ACTCAC)GATCCATACACCAGACTAACTCGGGAATAGT NGG 10 8 17 8 24 0 100 5 10
    240604222729_ktd3 TGCCCCATAGACCCGTTCTACACAGACAATGG(+ACTCAC)GATCCAGAGGGTCTGCGGCAAGAAGGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240604222727_aw6d AGTTATTGGACCTTCTTGCCGCAGACCCTCTG(+ACTCAC)GATCCCATTGTCTGTGTAGAACGGGTCTATG NGG 10 8 17 8 24 0 100 5 10