Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222725_lfj1 GTGACATCGAGATCAATTCAGGAGTCCAAGAG(+ACTCAC)GTACCTGTAAACTTAAACACAAGTCTGGACT NGG 10 8 17 8 24 0 100 5 10
    240604222724_e77a GATGCAGTCCAGACTTGTGTTTAAGTTTACAG(+ACTCAC)GTACCTCTTGGACTCCTGAATTGATCTCGAT NGG 10 8 17 8 24 0 100 5 10
    240604222722_rgcn GAACTAACTCACAGGCTCTTGTCAAAGTCTGG(+ACTCAC)GTACCAAATTTAGAAGTCTTTTTGATGTACC NGG 10 8 17 8 24 0 100 5 10
    240604222721_4370 AAGAGGGTACATCAAAAAGACTTCTAAATTTG(+ACTCAC)GTACCCAGACTTTGACAAGAGCCTGTGAGTT NGG 10 8 17 8 24 0 100 5 10
    240604222719_k0ed TCTACTTAATTAGCGACCAGCAAACACTAATG(+ACTCAC)GATCCATGGGGTAGGGTTTTTGGTTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240604222717_1w32 AACAACAACAAAACCAAAAACCCTACCCCATG(+ACTCAC)GATCCATTAGTGTTTGCTGGTCGCTAATTAA NGG 10 8 17 8 24 0 100 5 10
    240604222714_8uyn GTCATCCTTGTGATGGCATGCTCCTTTGCTGG(+ACTCAC)GTACCATGTAGGGCCATTTTTTTCCCCCTGC NGG 10 8 17 8 24 0 100 5 10
    240604222712_h1bl AAACTGCAGGGGGAAAAAAATGGCCCTACATG(+ACTCAC)GTACCCAGCAAAGGAGCATGCCATCACAAGG NGG 10 8 17 8 24 0 100 5 10
    240604222711_pu0z AAGTGCACGCATGTGCAGGAATGTCGCAGTGG(+ACTCAC)GATCCGGAGATCGCGGAGACCGTGGAGCCCG NGG 10 8 17 8 24 0 100 5 10
    240604222709_s6yc AGGAACGGGCTCCACGGTCTCCGCGATCTCCG(+ACTCAC)GATCCCACTGCGACATTCCTGCACATGCGTG NGG 10 8 17 8 24 0 100 5 10