Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222651_lpx0 ATAGTTAACCACATATCTAGAAATACCAGCAG(+ACTCAC)GATCCTGGAGGGAGGGGGGAGGGGGAGGGGA NGG 10 8 17 8 24 0 100 5 10
    240604222649_nvj0 CTCCTTCCCCTCCCCCTCCCCCCTCCCTCCAG(+ACTCAC)GATCCTGCTGGTATTTCTAGATATGTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240604222647_813n TAAACAACTAATGTGGAAGGCTGATGCAGGAG(+ACTCAC)GATCCATAGGAAACAGAAACAGCCCAAATGC NGG 10 8 17 8 24 0 100 5 10
    240604222645_0f38 GGGGGGCATTTGGGCTGTTTCTGTTTCCTATG(+ACTCAC)GATCCTCCTGCATCAGCCTTCCACATTAGTT NGG 10 8 17 8 24 0 100 5 10
    240604222643_8di1 CCACCTGGATCTCAGAACTTAATTCAAGTGGG(+ACTCAC)GTACCATATGTGATGGGTGTCTGTGATGGAG NGG 10 8 17 8 24 0 100 5 10
    240604222641_7dje CGTTTCTCCATCACAGACACCCATCACATATG(+ACTCAC)GTACCCCACTTGAATTAAGTTCTGAGATCCA NGG 10 8 17 8 24 0 100 5 10
    240604222640_46wz TAGGAGAAGTCATAATTAAATCCTGCAGTGGG(+ACTCAC)GTACCAGTGACCCCAGTCACGTTGGACTAAG NGG 10 8 17 8 24 0 100 5 10
    240604222638_vf0m AGCTTCTTAGTCCAACGTGACTGGGGTCACTG(+ACTCAC)GTACCCCACTGCAGGATTTAATTATGACTTC NGG 10 8 17 8 24 0 100 5 10
    240604222636_vfhr CACTTAATTCTCTGACCGGGCCAGCATCATGG(+ACTCAC)GTACCTGCTCCCCTGTGTACCCCATCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240604222635_4fwq AAACCTTAAGGATGGGGTACACAGGGGAGCAG(+ACTCAC)GTACCCATGATGCTGGCCCGGTCAGAGAATT NGG 10 8 17 8 24 0 100 5 10