Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604222912_j195 GAAGCCTTCATGAGTCTTCCACTCTCTTCTGG(+ACTCAC)GTACCGACGCAGAGATGGCATGTCACAGGAT NGG 10 8 17 8 24 0 100 5 10
    240604222911_58ql CCGGAATCCTGTGACATGCCATCTCTGCGTCG(+ACTCAC)GTACCCAGAAGAGAGTGGAAGACTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240604222909_fsyv GTTACCTCACAGACCACCACAGCCCACTCTGG(+ACTCAC)GTACCATCATCCCTGTCTCTGCCATTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240604222908_6p4m CTAGGGCAGGAATGGCAGAGACAGGGATGATG(+ACTCAC)GTACCCAGAGTGGGCTGTGGTGGTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240604222907_gmvc TTCTGCCAGTGTGTGTGACTATGTGAGTGTTG(+ACTCAC)GATCCCCTGACACTGGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240604222905_7j6f GCTTACAACTGTCTGTAACTCCAGTGTCAGGG(+ACTCAC)GATCCAACACTCACATAGTCACACACACTGG NGG 10 8 17 8 24 0 100 5 10
    240604222904_m9ch GAATGTAAACAGTTTTATTCAGAAACAGATAG(+ACTCAC)GTACCTGTTTGCATTACAGAATATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240604222902_nn19 AAACCAAGTTTTATATTCTGTAATGCAAACAG(+ACTCAC)GTACCTATCTGTTTCTGAATAAAACTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240604222901_zxe3 GGAGAAGAGTGGTTTGCCTTCTGAGGTATGAG(+ACTCAC)GATCCCTGGTCCAATCAGCAGGGTGAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240604222859_pnuy TTCCTAAACCTCACCCTGCTGATTGGACCAGG(+ACTCAC)GATCCTCATACCTCAGAAGGCAAACCACTCT NGG 10 8 17 8 24 0 100 5 10