Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604223528_hr64 CATCAGATGAGAACTCCATTAGCTGGCTAGAG(+ACTCAC)GATCCACATACTTCTCACCAATAGGACTGTA NGG 10 8 17 8 24 0 100 5 10
    240604223526_2nia AAAGTTACAGTCCTATTGGTGAGAAGTATGTG(+ACTCAC)GATCCTCTAGCCAGCTAATGGAGTTCTCATC NGG 10 8 17 8 24 0 100 5 10
    240604223524_x6ud ACTGTAATCTCGTTTCCATGCTGCCGAACCTG(+ACTCAC)GATCCCTATTTTAAACTATATTGATGGAATG NGG 10 8 17 8 24 0 100 5 10
    240604223522_mj1z GTGTGCATTCCATCAATATAGTTTAAAATAGG(+ACTCAC)GATCCAGGTTCGGCAGCATGGAAACGAGATT NGG 10 8 17 8 24 0 100 5 10
    240604223521_5i4r TTAAGCTGCAAATCTTCTCAGGTGTTCATATG(+ACTCAC)GTACCTATCCATAGATACTCACATAGTAAAA NGG 10 8 17 8 24 0 100 5 10
    240604223520_rb77 TAGGGTTTTACTATGTGAGTATCTATGGATAG(+ACTCAC)GTACCATATGAACACCTGAGAAGATTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240604223518_bit3 AAGATAAGGATAAGGAAGTTGTAGTTTATATG(+ACTCAC)GATCCCAAAGTCTTGTGGTTTTGCTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240604223517_pkbo AAACTGGGAACAGCAAAACCACAAGACTTTGG(+ACTCAC)GATCCATATAAACTACAACTTCCTTATCCTT NGG 10 8 17 8 24 0 100 5 10
    240604223516_qltc CCAGCCCTTTGGAGTAGCTCAAATCATGTGTG(+ACTCAC)GATCCCAAACATTGAAACAAGAAGCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240604223514_3fv9 TTAATGCTCCAGCTTCTTGTTTCAATGTTTGG(+ACTCAC)GATCCACACATGATTTGAGCTACTCCAAAGG NGG 10 8 17 8 24 0 100 5 10