Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224546_ytxn CTTCGGTACCTGGGATTACAGATGCTTCTCAG(+ACTCAC)GATCCCAGCCATTCTGCTTTCATTATTAAAG NGG 10 8 17 8 24 0 100 5 10
    240604224544_0ez7 GTAACCTTTAATAATGAAAGCAGAATGGCTGG(+ACTCAC)GATCCTGAGAAGCATCTGTAATCCCAGGTAC NGG 10 8 17 8 24 0 100 5 10
    240604224543_1jpb TGAACCTGAGACCCTCCTGTGTCAACCCTTAG(+ACTCAC)GTACCTGGGATTGCAGATGCTTCTCACTATC NGG 10 8 17 8 24 0 100 5 10
    240604224540_cnru GCTGGGATAGTGAGAAGCATCTGCAATCCCAG(+ACTCAC)GTACCTAAGGGTTGACACAGGAGGGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240604224538_da8g GAGCATGCAGCTAAGCAGATATGAGCAGGAGG(+ACTCAC)GATCCCATGTAAGAAAGTAAGACACCAGAGG NGG 10 8 17 8 24 0 100 5 10
    240604224536_fuv0 AAAGTCCTCTGGTGTCTTACTTTCTTACATGG(+ACTCAC)GATCCCTCCTGCTCATATCTGCTTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240604224535_2ogi GCTGTCTTCAGACATACCAGAAGAGGACATAG(+ACTCAC)GATCCTCATTTTATGTGGTTGGTAGGAATTG NGG 10 8 17 8 24 0 100 5 10
    240604224534_zl7p GAGTTCAATTCCTACCAACCACATAAAATGAG(+ACTCAC)GATCCTATGTCCTCTTCTGGTATGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240604224532_er4y CCTAGCTGGGCTCGACCACTTAGCTGGTGAAG(+ACTCAC)GATCCTTCCGAGCCCCCAACCCTACCCCAGA NGG 10 8 17 8 24 0 100 5 10
    240604224531_l4hm ATCGCTCTGGGGTAGGGTTGGGGGCTCGGAAG(+ACTCAC)GATCCTTCACCAGCTAAGTGGTCGAGCCCAG NGG 10 8 17 8 24 0 100 5 10