Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224602_lc3v ACAAAGACAAGGACCAGAAGCAAATACTGGTG(+ACTCAC)GATCCAGATGTTTGAAATCTTTGTATCAAAA NGG 10 8 17 8 24 0 100 5 10
    240604224601_h4me ACACGTTTTGATACAAAGATTTCAAACATCTG(+ACTCAC)GATCCACCAGTATTTGCTTCTGGTCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240604224559_uxky GCTCAACAATACAGAGAATATGTGAACCTGAG(+ACTCAC)GTACCCTGACTCAAATTTCACAACCACAATG NGG 10 8 17 8 24 0 100 5 10
    240604224557_y5dr GGTTTCATTGTGGTTGTGAAATTTGAGTCAGG(+ACTCAC)GTACCTCAGGTTCACATATTCTCTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240604224556_yv72 AAGACATATACATATATGTTAATCAAATATTG(+ACTCAC)GTACCAGAGTACACACTGTGTTTGCATGATT NGG 10 8 17 8 24 0 100 5 10
    240604224555_4yn3 GAGAAAATCATGCAAACACAGTGTGTACTCTG(+ACTCAC)GTACCAATATTTGATTAACATATATGTATAT NGG 10 8 17 8 24 0 100 5 10
    240604224553_zkoq CCAGGTCAGTTGTAGGAATTCTTGAGTATCAG(+ACTCAC)GATCCATCGATGTGTAGACAAAACTGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240604224552_lamf CACTCTAAAACAGTTTTGTCTACACATCGATG(+ACTCAC)GATCCTGATACTCAAGAATTCCTACAACTGA NGG 10 8 17 8 24 0 100 5 10
    240604224550_m0b1 TGAACCTGAGACCCTCCTGTGTCAACCCTTCG(+ACTCAC)GTACCTGGGATTACAGATGCTTCTCAGGATC NGG 10 8 17 8 24 0 100 5 10
    240604224548_0jal GCTGGGATCCTGAGAAGCATCTGTAATCCCAG(+ACTCAC)GTACCGAAGGGTTGACACAGGAGGGTCTCAG NGG 10 8 17 8 24 0 100 5 10