Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604224651_gnb9 AAGTGACTCTTCGGGTGGCTTTCCCTCTCAGG(+ACTCAC)GTACCTAGAGGTGTGAATTTGAAGCCATGCT NGG 10 8 17 8 24 0 100 5 10
    240604224650_q95i TTCCCAGCATGGCTTCAAATTCACACCTCTAG(+ACTCAC)GTACCCTGAGAGGGAAAGCCACCCGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240604224648_p2aa TATTCACACTCCTAATCAAACGTGCACTATGG(+ACTCAC)GTACCTAGTTTTTCTCTTAAGAACATACATG NGG 10 8 17 8 24 0 100 5 10
    240604224646_z90w ACTTACATGTATGTTCTTAAGAGAAAAACTAG(+ACTCAC)GTACCCATAGTGCACGTTTGATTAGGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240604224645_iupz TTATTCAAATTTTGATTATTTTAAAAATAAAG(+ACTCAC)GATCCATTCTTACCGAAAGGGACACTACATC NGG 10 8 17 8 24 0 100 5 10
    240604224644_um2l TTAAAGATGTAGTGTCCCTTTCGGTAAGAATG(+ACTCAC)GATCCTTTATTTTTAAAATAATCAAAATTTG NGG 10 8 17 8 24 0 100 5 10
    240604224642_ru5l CTCCACTTCCCATCTGACCGCTGATGTGCTGG(+ACTCAC)GATCCCAGAGCATACTCCTGAGCCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240604224640_5pj8 GTCATAAAGCCAGGCTCAGGAGTATGCTCTGG(+ACTCAC)GATCCCAGCACATCAGCGGTCAGATGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240604224639_b82g TATGTTAATATGATGAAATAAAATCAGAACTG(+ACTCAC)GTACCTATTTGTACGTAAGTGTGAGACGTGG NGG 10 8 17 8 24 0 100 5 10
    240604224637_pcaj AGAAGCCACGTCTCACACTTACGTACAAATAG(+ACTCAC)GTACCAGTTCTGATTTTATTTCATCATATTA NGG 10 8 17 8 24 0 100 5 10