Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221421_zsvu AGTGAGGGCTCTCAGGTCAGCCACAGAACAGG(+ACTCAC)GATCCACTTAGCTCACTGCCTACTGCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240604221420_op0h GCCGGGCTCGGCAGTAGGCAGTGAGCTAAGTG(+ACTCAC)GATCCCTGTTCTGTGGCTGACCTGAGAGCCC NGG 10 8 17 8 24 0 100 5 10
    240604221419_2gwt GCCCTCATGATGACAGGGTGCATTTGTAACAG(+ACTCAC)GTACCTTGCACACAGTCAGCCCATCTCACAG NGG 10 8 17 8 24 0 100 5 10
    240604221417_z1vd TCCTACTGTGAGATGGGCTGACTGTGTGCAAG(+ACTCAC)GTACCTGTTACAAATGCACCCTGTCATCATG NGG 10 8 17 8 24 0 100 5 10
    240604221416_3i8o ATCAGGTTAGCACTGGCTGTAGGCTGGTCTGG(+ACTCAC)GATCCTAACAGAATGGCAAGAGGTGAGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604221414_wtp3 ACTGCTTCCCTCACCTCTTGCCATTCTGTTAG(+ACTCAC)GATCCCAGACCAGCCTACAGCCAGTGCTAAC NGG 10 8 17 8 24 0 100 5 10
    240604221412_z39a AAAGAATTTAAAGGGGGGGGGGGGGTTTCCAG(+ACTCAC)GTACCCCTGTCCAGGCTTAAACAAAGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240604221410_qkme AAGGCTCTTGCTTTGTTTAAGCCTGGACAGGG(+ACTCAC)GTACCTGGAAACCCCCCCCCCCCCTTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240604221407_6934 TTCACATCTGAGACAAATAGAAAGCAGCCCAG(+ACTCAC)GTACCAATTTGCTCTTCAGTGTCAGTCAGAG NGG 10 8 17 8 24 0 100 5 10
    240604221406_w4o6 TTTTCCTCTGACTGACACTGAAGAGCAAATTG(+ACTCAC)GTACCTGGGCTGCTTTCTATTTGTCTCAGAT NGG 10 8 17 8 24 0 100 5 10