Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221331_c4nh CACCAAGAGCAGAGGTACTGCTGGGATTATAG(+ACTCAC)GATCCTACAATTCTCACTGGACCTGGAAGGG NGG 10 8 17 8 24 0 100 5 10
    240604221330_2ye7 GGGTGCCCTTCCAGGTCCAGTGAGAATTGTAG(+ACTCAC)GATCCTATAATCCCAGCAGTACCTCTGCTCT NGG 10 8 17 8 24 0 100 5 10
    240604221329_hn0j ATAAGTAATATGCCATTTCACCAAATACACTG(+ACTCAC)GATCCCTCTCACAAACACTACCACACTTTAT NGG 10 8 17 8 24 0 100 5 10
    240604221327_l9ng AGTGCATAAAGTGTGGTAGTGTTTGTGAGAGG(+ACTCAC)GATCCAGTGTATTTGGTGAAATGGCATATTA NGG 10 8 17 8 24 0 100 5 10
    240604221326_tunu GGTGACACACTAAAGGAGAGCCCAGACATTAG(+ACTCAC)GATCCAAAAAGCAGTTCAACAAACTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604221325_vlj6 CTGAAAAAAAAAGTTTGTTGAACTGCTTTTTG(+ACTCAC)GATCCTAATGTCTGGGCTCTCCTTTAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240604221323_a9nw TGCACACTGCTACATCTGTGCGGGGTGCCTAG(+ACTCAC)GTACCTTGGCAGGAAAGCAAGAACAGTCTAG NGG 10 8 17 8 24 0 100 5 10
    240604221321_x2z7 CACTTCTAGACTGTTCTTGCTTTCCTGCCAAG(+ACTCAC)GTACCTAGGCACCCCGCACAGATGTAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240604221319_7q8z TAACACAGAAGGGGAAAGACACTGTTGAGAGG(+ACTCAC)GATCCCAGAGAGGGAGGGAGAGAACGAAGGA NGG 10 8 17 8 24 0 100 5 10
    240604221317_s1rz CTAGTTCCTTCGTTCTCTCCCTCCCTCTCTGG(+ACTCAC)GATCCCTCTCAACAGTGTCTTTCCCCTTCTG NGG 10 8 17 8 24 0 100 5 10