Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221231_w8av ATGAAGTGGACACTTTTCTAGACAGATACTAG(+ACTCAC)GTACCAAAGTTAAATCAGGATCAGATTAACG NGG 10 8 17 8 24 0 100 5 10
    240604221229_h5op TAAATCGTTAATCTGATCCTGATTTAACTTTG(+ACTCAC)GTACCTAGTATCTGTCTAGAAAAGTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240604221228_8nvr TGCTCCTAGTCACCCATTGGATGCAGCACAGG(+ACTCAC)GTACCCAATGAAGGAGCTAAAGAAAGTACCT NGG 10 8 17 8 24 0 100 5 10
    240604221226_m0sf TCTTTAGGTACTTTCTTTAGCTCCTTCATTGG(+ACTCAC)GTACCCTGTGCTGCATCCAATGGGTGACTAG NGG 10 8 17 8 24 0 100 5 10
    240604221225_0qzh CATAGTGAGGAAATGAAATGATGCACTTAATG(+ACTCAC)GTACCATCTCACTGTCAAGATAAAATACCCA NGG 10 8 17 8 24 0 100 5 10
    240604221224_gtxy GCAGCTGGGTATTTTATCTTGACAGTGAGATG(+ACTCAC)GTACCATTAAGTGCATCATTTCATTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240604221223_54o1 ATTGTTGAAAGTCAAACTTATTGTGAGTTAAG(+ACTCAC)GATCCATAAAATTCTTCTGTTGCAGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240604221222_uvf6 AGTATCCCTAACTGCAACAGAAGAATTTTATG(+ACTCAC)GATCCTTAACTCACAATAAGTTTGACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240604221220_saek AATCCACTCAGGACCACACAGTAACACATGTG(+ACTCAC)GATCCTGGCACCCCCACTTCTGCCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240604221218_3rkx CTGGAAGTTGGAGGCAGAAGTGGGGGTGCCAG(+ACTCAC)GATCCACATGTGTTACTGTGTGGTCCTGAGT NGG 10 8 17 8 24 0 100 5 10