Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221141_rq4c AGATGTTGCATGCACCAACTGAGCTATCATTG(+ACTCAC)GATCCAAGTGGTCAGCGCTGAAATCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240604221140_1uuk ACGGAAGGAGAGATTTCAGCGCTGACCACTTG(+ACTCAC)GATCCAATGATAGCTCAGTTGGTGCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240604221137_po53 CCCTTCCTGAGGACTGTAACTCCAGCTCTGGG(+ACTCAC)GATCCAGTGTCCTCTCGTGCCCTCCAGAGCA NGG 10 8 17 8 24 0 100 5 10
    240604221136_bxeq GTGCATGCTCTGGAGGGCACGAGAGGACACTG(+ACTCAC)GATCCCCAGAGCTGGAGTTACAGTCCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240604221135_fx0t CGCTGCTTGTATATGAAAATAAAAGGCATACG(+ACTCAC)GTACCCCTGAGACAAAAGCCGAGGGGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240604221132_gokd GAATAACACCCCCCTCGGCTTTTGTCTCAGGG(+ACTCAC)GTACCGTATGCCTTTTATTTTCATATACAAG NGG 10 8 17 8 24 0 100 5 10
    240604221131_waie CTGTGAGGTCAAGGCATCGAGCGGCATAGTAG(+ACTCAC)GATCCTGTCTCAAGCACACCGGCTAGAGTGG NGG 10 8 17 8 24 0 100 5 10
    240604221129_jp0b AGCTACCACTCTAGCCGGTGTGCTTGAGACAG(+ACTCAC)GATCCTACTATGCCGCTCGATGCCTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240604221128_q88b CAGGAGACCAATCCTGCCAGGTGGGCAAGCTG(+ACTCAC)GATCCGGGTAACAGCTATTTGCAAAGAACAA NGG 10 8 17 8 24 0 100 5 10
    240604221125_v437 GTTGGTTGTTCTTTGCAAATAGCTGTTACCCG(+ACTCAC)GATCCAGCTTGCCCACCTGGCAGGATTGGTC NGG 10 8 17 8 24 0 100 5 10