Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221109_5fio GGAGGGGAAACCCCTCCCACCCACCAAGTTTG(+ACTCAC)GATCCTGGACCTCAGCTTGAACAGTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240604221107_ns2l CTCACTGGAATACTGTTCAAGCTGAGGTCCAG(+ACTCAC)GATCCAAACTTGGTGGGTGGGAGGGGTTTCC NGG 10 8 17 8 24 0 100 5 10
    240604221105_cjdj AAATGCTATGACTTTAAGGGTCTTGGGGGGAG(+ACTCAC)GATCCATGACCTAAACACAGTCGTTTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604221104_cifq AAAAAAAAAAAAACGACTGTGTTTAGGTCATG(+ACTCAC)GATCCTCCCCCCAAGACCCTTAAAGTCATAG NGG 10 8 17 8 24 0 100 5 10
    240604221102_lbcc AATAAAAGTAAGAAAATATGCTCAATGCTGAG(+ACTCAC)GTACCTTAAAAAAACAAAAGTTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240604221101_5drl TAAAATATTTTATAAACTTTTGTTTTTTTAAG(+ACTCAC)GTACCTCAGCATTGAGCATATTTTCTTACTT NGG 10 8 17 8 24 0 100 5 10
    240604221059_gro2 TGCTCAACCTTATTTATATCACTGACTGGCTG(+ACTCAC)GATCCAAAGTCACACCTCCACATATTAGTGA NGG 10 8 17 8 24 0 100 5 10
    240604221058_ig4q TTTATTCACTAATATGTGGAGGTGTGACTTTG(+ACTCAC)GATCCAGCCAGTCAGTGATATAAATAAGGTT NGG 10 8 17 8 24 0 100 5 10
    240604221057_cjsb ACTGTGGGTGCTTGAGGAGGCCAGAGGCATAG(+ACTCAC)GATCCCTCTGGAGCATGAATTACAAGTGGCT NGG 10 8 17 8 24 0 100 5 10
    240604221055_d8c3 CTGACAGCCACTTGTAATTCATGCTCCAGAGG(+ACTCAC)GATCCTATGCCTCTGGCCTCCTCAAGCACCC NGG 10 8 17 8 24 0 100 5 10