Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221842_bcyd CACACTCTCCAACCTTCTTCCTGGGAGCCGTG(+ACTCAC)GTACCACCAGGCATCCTTGGGATGGAAACCA NGG 10 8 17 8 24 0 100 5 10
    240604221841_r1zz GCCACCCAGACTGTGGTAGCTTGGCATGAATG(+ACTCAC)GTACCGGCTGTTCTTATGAGTGTGAATGTGT NGG 10 8 17 8 24 0 100 5 10
    240604221839_07r7 CACATACACATTCACACTCATAAGAACAGCCG(+ACTCAC)GTACCATTCATGCCAAGCTACCACAGTCTGG NGG 10 8 17 8 24 0 100 5 10
    240604221837_l23o CCTCGCCCCTGTGGGAAGGCAGGGCCAAAAAG(+ACTCAC)GTACCACATTCTCACTCAAAAAAAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240604221836_8z7z TTGGAGTTTGTTTTTTTTTGAGTGAGAATGTG(+ACTCAC)GTACCTTTTTGGCCCTGCCTTCCCACAGGGG NGG 10 8 17 8 24 0 100 5 10
    240604221834_1yn7 TTTATAGACCTCTTTCAGCTATTTTAATAAAG(+ACTCAC)GATCCTTTGGAATTTATCCCCAGCCGATGCT NGG 10 8 17 8 24 0 100 5 10
    240604221832_d7mw AAAACAGCATCGGCTGGGGATAAATTCCAAAG(+ACTCAC)GATCCTTTATTAAAATAGCTGAAAGAGGTCT NGG 10 8 17 8 24 0 100 5 10
    240604221831_5c6l ATGTAGTTCTTGTGCACAGGTGCCACTAGCTG(+ACTCAC)GTACCCTAGGCCTAGTCCCCAACCCAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240604221829_ut76 CACCTCCTTTTGGGTTGGGGACTAGGCCTAGG(+ACTCAC)GTACCAGCTAGTGGCACCTGTGCACAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240604221828_awrx ATGTATATCTCATTACCCTCATCCCCATCTTG(+ACTCAC)GTACCTGGGATCTTGGTTCTCACAAGTAATT NGG 10 8 17 8 24 0 100 5 10