Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604220948_2rv0 TCTTAAGTGAGCACTAACTTGTTACAGTGCTG(+ACTCAC)GATCCAGCCAGCCCCAGAGGCCCAATCTCAT NGG 10 8 17 8 24 0 100 5 10
    240604220946_etkb AGTTAATGAGATTGGGCCTCTGGGGCTGGCTG(+ACTCAC)GATCCAGCACTGTAACAAGTTAGTGCTCACT NGG 10 8 17 8 24 0 100 5 10
    240604220945_m1k8 AAAAGGACACATAGTATATAACTCCACTGTTG(+ACTCAC)GTACCAACAATATTTACTGCAGAAAACCACG NGG 10 8 17 8 24 0 100 5 10
    240604220944_48xb GTTTCCGTGGTTTTCTGCAGTAAATATTGTTG(+ACTCAC)GTACCAACAGTGGAGTTATATACTATGTGTC NGG 10 8 17 8 24 0 100 5 10
    240604220942_3ssx AAGTAGAAAATCATCTTTAAAAGACAGCCAAG(+ACTCAC)GTACCATCACATCTCCCTGAAAGTAAGACAG NGG 10 8 17 8 24 0 100 5 10
    240604220939_ym3s CTGTTCTGTCTTACTTTCAGGGAGATGTGATG(+ACTCAC)GTACCTTGGCTGTCTTTTAAAGATGATTTTC NGG 10 8 17 8 24 0 100 5 10
    240604220937_eobl ATTGATTCCTACTTTATTATCTTAAAGACTAG(+ACTCAC)GATCCTGTTATTTTTCTTTAAATGTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240604220936_fkgx AGTGCCTGTGAACATTTAAAGAAAAATAACAG(+ACTCAC)GATCCTAGTCTTTAAGATAATAAAGTAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604220935_x4e8 AAGGCCTACCTCCAGGGATGCAGCTCCTACAG(+ACTCAC)GATCCCAGAATGAAGTTGACTCGAATATTAC NGG 10 8 17 8 24 0 100 5 10
    240604220933_5e9s CTGATGTAATATTCGAGTCAACTTCATTCTGG(+ACTCAC)GATCCTGTAGGAGCTGCATCCCTGGAGGTAG NGG 10 8 17 8 24 0 100 5 10