Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221005_x1cr AATTATCTGCAACCCCCGTCCTAGAGGATCTG(+ACTCAC)GTACCCACCTATGTGGGCATTGCAAGCACGG NGG 10 8 17 8 24 0 100 5 10
    240604221003_p2lx GTGCCCCGTGCTTGCAATGCCCACATAGGTGG(+ACTCAC)GTACCAGATCCTCTAGGACGGGGGTTGCAGA NGG 10 8 17 8 24 0 100 5 10
    240604221001_nqi4 TAAAAAAGGACAAATTTGGCAGAACTTGATTG(+ACTCAC)GATCCAGGACCTCCCACATGTTAAGCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240604220959_xibh TGGAGTGCTGGCTTAACATGTGGGAGGTCCTG(+ACTCAC)GATCCAATCAAGTTCTGCCAAATTTGTCCTT NGG 10 8 17 8 24 0 100 5 10
    240604220958_vkso TTGTTTGTTTGTTTGTTTGCTTTCTAGACAGG(+ACTCAC)GTACCCCTTTGTAGCCCTGACAGTACTAGAA NGG 10 8 17 8 24 0 100 5 10
    240604220955_l7gm GTGAGTTCTAGTACTGTCAGGGCTACAAAGGG(+ACTCAC)GTACCCTGTCTAGAAAGCAAACAAACAAACA NGG 10 8 17 8 24 0 100 5 10
    240604220954_mhj1 TAGCCACACTGTACCCACTATGGTACTCAATG(+ACTCAC)GTACCAGATGAAATGTTGAGTACTTCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240604220953_bhmo TGTTTAGGGGGAAGTACTCAACATTTCATCTG(+ACTCAC)GTACCATTGAGTACCATAGTGGGTACAGTGT NGG 10 8 17 8 24 0 100 5 10
    240604220951_xonm CAGGATAATGATACCATAAGTGTCATGGGAGG(+ACTCAC)GATCCTGGTGCACAAAAACTGTTTAAAGATA NGG 10 8 17 8 24 0 100 5 10
    240604220949_ym3q GGCCCTATCTTTAAACAGTTTTTGTGCACCAG(+ACTCAC)GATCCCTCCCATGACACTTATGGTATCATTA NGG 10 8 17 8 24 0 100 5 10