Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221217_50ys CTCTACAAAGATGTGATGCTGGAGATCTTCAG(+ACTCAC)GATCCTCACTACTATAGGCTACACCTGGGAA NGG 10 8 17 8 24 0 100 5 10
    240604221216_qppe ATGTCTTCCCAGGTGTAGCCTATAGTAGTGAG(+ACTCAC)GATCCTGAAGATCTCCAGCATCACATCTTTG NGG 10 8 17 8 24 0 100 5 10
    240604221214_n5r8 AATAGCCACATGGGTACTGAGAATTAATCCTG(+ACTCAC)GATCCTCTGAAAATGCCAGGTAATCAATGAG NGG 10 8 17 8 24 0 100 5 10
    240604221213_nph7 GACGGCTCATTGATTACCTGGCATTTTCAGAG(+ACTCAC)GATCCAGGATTAATTCTCAGTACCCATGTGG NGG 10 8 17 8 24 0 100 5 10
    240604221212_cbtk ATGGCATTGTGCAGGCAGATTTGGTGCTGGAG(+ACTCAC)GATCCAAAGGCAGCCAGACAATCTCCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240604221209_nb19 CCAGTTCAGAGGAGATTGTCTGGCTGCCTTTG(+ACTCAC)GATCCTCCAGCACCAAATCTGCCTGCACAAT NGG 10 8 17 8 24 0 100 5 10
    240604221207_s0pf TTCTTTGGGTTTGTCGTTGGATTTGGAGCGAG(+ACTCAC)GATCCATTTTCTCTTTACTCTGTCATTTGTC NGG 10 8 17 8 24 0 100 5 10
    240604221206_efn7 ACAGTGACAAATGACAGAGTAAAGAGAAAATG(+ACTCAC)GATCCTCGCTCCAAATCCAACGACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240604221203_7kjf TCACCTAGAGACCCTGTGTAGATTTAACCAAG(+ACTCAC)GTACCAAGAAAGTCCTGATGTTACCACAAGG NGG 10 8 17 8 24 0 100 5 10
    240604221201_xg8s GTGGTCCTTGTGGTAACATCAGGACTTTCTTG(+ACTCAC)GTACCTTGGTTAAATCTACACAGGGTCTCTA NGG 10 8 17 8 24 0 100 5 10