Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221259_khy5 CGAGTGGTCATGCCCTCCTTTTCATTCATCTG(+ACTCAC)GTACCAACACCAGAGGGAGAGCATACAGGAC NGG 10 8 17 8 24 0 100 5 10
    240604221258_p4gk GGGAAGTCCTGTATGCTCTCCCTCTGGTGTTG(+ACTCAC)GTACCAGATGAATGAAAAGGAGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240604221256_znkc TGTGGAAGAAGCTTGCTGCCTGAGGGCTGAGG(+ACTCAC)GATCCATAGGATCTCAATGAGGCTTGCAGTT NGG 10 8 17 8 24 0 100 5 10
    240604221254_jh3c CATGTAACTGCAAGCCTCATTGAGATCCTATG(+ACTCAC)GATCCCTCAGCCCTCAGGCAGCAAGCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240604221253_9niw CATCAAGCAACTCTGAGTCTCTCTGGCTTCTG(+ACTCAC)GATCCTGCTACAATATATGTGGGCAGGAACA NGG 10 8 17 8 24 0 100 5 10
    240604221252_3p4k CCCTTTGTTCCTGCCCACATATATTGTAGCAG(+ACTCAC)GATCCAGAAGCCAGAGAGACTCAGAGTTGCT NGG 10 8 17 8 24 0 100 5 10
    240604221250_kslz ACGGCCAAGCCATCCAAAACTAAGGGAAGTGG(+ACTCAC)GATCCCTAAGAGGTGAACCCTTAAGTGTTGT NGG 10 8 17 8 24 0 100 5 10
    240604221249_5q5h TGGGGACAACACTTAAGGGTTCACCTCTTAGG(+ACTCAC)GATCCCACTTCCCTTAGTTTTGGATGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240604221247_027l TTTATATTCCAATTATGCCAACAAAGGGCAAG(+ACTCAC)GTACCAGGAAAAGCATTAAACTATATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240604221246_hzxn GTGTTAAAAAATATAGTTTAATGCTTTTCCTG(+ACTCAC)GTACCTTGCCCTTTGTTGGCATAATTGGAAT NGG 10 8 17 8 24 0 100 5 10