Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240604221404_4t9k GGGTGGGTGAGTGCTGGCATGGACGTCTCTAG(+ACTCAC)GATCCTCTACAAACAGGCCCAGTTCACAAGC NGG 10 8 17 8 24 0 100 5 10
    240604221402_vzkw TCCTGGCTTGTGAACTGGGCCTGTTTGTAGAG(+ACTCAC)GATCCTAGAGACGTCCATGCCAGCACTCACC NGG 10 8 17 8 24 0 100 5 10
    240604221400_pbsj GTGCTGTGTTGGGAAGCTTTGGAGGTTCCTAG(+ACTCAC)GTACCTGGTATCTGGCTCCCAGCAGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240604221358_csi2 AACAGGAGCTGCTGCTGGGAGCCAGATACCAG(+ACTCAC)GTACCTAGGAACCTCCAAAGCTTCCCAACAC NGG 10 8 17 8 24 0 100 5 10
    240604221356_h4kj TGTTTAGACACATTTTACTAGGCACCTCCGTG(+ACTCAC)GATCCTTTACAGTGTTCTTGTGACAAGCTAG NGG 10 8 17 8 24 0 100 5 10
    240604221355_004c CCACACTAGCTTGTCACAAGAACACTGTAAAG(+ACTCAC)GATCCACGGAGGTGCCTAGTAAAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240604221354_fnju GCCATTTCCTTTCCCAACAGAACTCCTCATAG(+ACTCAC)GATCCCCTGTCCTTTCCCTGCCCACCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240604221352_vk33 GAGTACAAAAGGTGGGCAGGGAAAGGACAGGG(+ACTCAC)GATCCTATGAGGAGTTCTGTTGGGAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240604221351_n39g ACACAACTCCCCCACAAAAATCACTGGAGTAG(+ACTCAC)GATCCCATTAACTACACTCCTCAAATAATCA NGG 10 8 17 8 24 0 100 5 10
    240604221349_ff4i CAGACTGATTATTTGAGGAGTGTAGTTAATGG(+ACTCAC)GATCCTACTCCAGTGATTTTTGTGGGGGAGT NGG 10 8 17 8 24 0 100 5 10