Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160244_3i5n GTTCACAATGTGCGTATTTATAAGGTAGTTAG(+AGGACA)GTACCATTTGTAAGGTAAGTCCTTTAACATT NGG 10 8 17 8 24 0 100 5 10
    240613160242_6yz4 TATAGAATGTTAAAGGACTTACCTTACAAATG(+AGGACA)GTACCTAACTACCTTATAAATACGCACATTG NGG 10 8 17 8 24 0 100 5 10
    240613160241_8cx7 GAACTCTCTATTTTCCAGAATGCTTTGTCAAG(+AGGACA)GATCCTCTGTGGTCTGTGGCAGTTTTATCAG NGG 10 8 17 8 24 0 100 5 10
    240613160240_dods GCTTCCTGATAAAACTGCCACAGACCACAGAG(+AGGACA)GATCCTTGACAAAGCATTCTGGAAAATAGAG NGG 10 8 17 8 24 0 100 5 10
    240613160238_dtt3 AGGACAGAAGAGGAGAAAGGGAAGGCCTGAGG(+AGGACA)GTACCTCAGCCGCCAGCTACCTTCACTTTGA NGG 10 8 17 8 24 0 100 5 10
    240613160237_1iiw AGACTTCAAAGTGAAGGTAGCTGGCGGCTGAG(+AGGACA)GTACCCTCAGGCCTTCCCTTTCTCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613160235_3ht2 GTAAACAGCAAGAGGCTTTATTGGGAACACGG(+AGGACA)GTACCCGGGCGACTCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613160233_aa7n GCGCCAGTCCTCCGATAGACTGAGTCGCCCGG(+AGGACA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613160232_x94l TTATGCAGATGCTCTTGCTGTGTTCCTCAAAG(+AGGACA)GATCCAAGCCATAAAATTCTTTATGCATGTC NGG 10 8 17 8 24 0 100 5 10
    240613160230_icdm ACTGTGACATGCATAAAGAATTTTATGGCTTG(+AGGACA)GATCCTTTGAGGAACACAGCAAGAGCATCTG NGG 10 8 17 8 24 0 100 5 10