Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160030_9684 CTTCCAAGGGATGCACACCCAGCTGCTTCATG(+AGGACA)GTACCCGCAGGATGCAAGACAGTTTGACTTT NGG 10 8 17 8 24 0 100 5 10
    240613160029_yy9v GAGTGGAGGTGCCTGCAGGGTCAGAGGTATTG(+AGGACA)GATCCCTCTGGAGCTGGAGTTACAGTCAGTT NGG 10 8 17 8 24 0 100 5 10
    240613160028_r7ou CTTACAACTGACTGTAACTCCAGCTCCAGAGG(+AGGACA)GATCCAATACCTCTGACCCTGCAGGCACCTC NGG 10 8 17 8 24 0 100 5 10
    240613160026_x39i CCTGCCTCATGGCCTTGCTCTGTAACTACCTG(+AGGACA)GTACCCCTTCTAGACTTCCTTTAGGCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613160024_fh1c AAAGGAGGCTGCCTAAAGGAAGTCTAGAAGGG(+AGGACA)GTACCAGGTAGTTACAGAGCAAGGCCATGAG NGG 10 8 17 8 24 0 100 5 10
    240613160022_tyce GGTGGCAGCCTCAGGATCTTGCCTTAGTCCTG(+AGGACA)GTACCCAGATCTGTTCTCCTGAGACTCATCC NGG 10 8 17 8 24 0 100 5 10
    240613160021_6o41 CTCTTGGATGAGTCTCAGGAGAACAGATCTGG(+AGGACA)GTACCAGGACTAAGGCAAGATCCTGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613160019_x95a CCAGCCTCCCATAAGGGTCAATACAACGCCTG(+AGGACA)GATCCCCAAACCTGTGCTCAGGAGGAAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613160017_xi48 AGCCCCAGCTTCCTCCTGAGCACAGGTTTGGG(+AGGACA)GATCCAGGCGTTGTATTGACCCTTATGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613160015_vu60 CCCTGATGGAGGAGGCCAGGGCTCAGAGCAGG(+AGGACA)GTACCCACTGTGGATCTTTGTCTGTGAACCG NGG 10 8 17 8 24 0 100 5 10