Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613160125_uhhi ATCGAGAAGCCATCCTGGGATGAAGCCTCTGG(+AGGACA)GATCCATGTCTCTCAGCTCTCACCCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240613160124_gn1o ATGGCTGCAGTGGGTGAGAGCTGAGAGACATG(+AGGACA)GATCCCAGAGGCTTCATCCCAGGATGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613160123_hkcs TAAATACCTGAGCCCCCTTTTTGTTCTTCACG(+AGGACA)GATCCTTGGTCTTAATGTTGAAACACCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613160121_ljgx GAAAGATTAGGTGTTTCAACATTAAGACCAAG(+AGGACA)GATCCGTGAAGAACAAAAAGGGGGCTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613160120_bbxb TAAATACCTGAGCCCCCTTTTTGTTCTTCACG(+AGGACA)GATCCTTGGTCTTAATGTTGAAACACCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613160118_92nx GAAAGATTAGGTGTTTCAACATTAAGACCAAG(+AGGACA)GATCCGTGAAGAACAAAAAGGGGGCTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613160116_9oih GTGGTTAAAGAGCACTGATTGCTCTTCCAGAG(+AGGACA)GATCCGGGTTCAATTCCAGCATCCATATGGC NGG 10 8 17 8 24 0 100 5 10
    240613160113_0znp GAGCTGCCATATGGATGCTGGAATTGAACCCG(+AGGACA)GATCCTCTGGAAGAGCAATCAGTGCTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613160112_01hu AACCTGGGAATAGTCAAGAATATTGTCATCTG(+AGGACA)GATCCCATTTAATTTATGGAATCTTTTATGG NGG 10 8 17 8 24 0 100 5 10
    240613160110_xluv TTTGACCATAAAAGATTCCATAAATTAAATGG(+AGGACA)GATCCAGATGACAATATTCTTGACTATTCCC NGG 10 8 17 8 24 0 100 5 10