Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155922_vkiy GGTTCACAGCCATCTATGACTTCAGTTCCCGG(+AGGACA)GATCCAGTGCTTTCTTCTGGCCTTCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613155921_q36z AGAAACCTACTTGCACATTCTCAGTACACTTG(+AGGACA)GATCCAAAATGTCTACACTTGATGCTCCGGC NGG 10 8 17 8 24 0 100 5 10
    240613155920_ate3 CTTGGGCCGGAGCATCAAGTGTAGACATTTTG(+AGGACA)GATCCAAGTGTACTGAGAATGTGCAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240613155918_lfrj GTTCACTTCCTCAATAAGGGCCTGATTTGGGG(+AGGACA)GTACCAACCTTCTCTCTCCCATTGACCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613155916_gc71 CAGAGCACTGGTCAATGGGAGAGAGAAGGTTG(+AGGACA)GTACCCCCAAATCAGGCCCTTATTGAGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613155915_o43y CCAAGCATGGGGCCCAAGGGAGGAGGGATCAG(+AGGACA)GATCCTAATTTTGCTCAGAGGAAGTGAACTT NGG 10 8 17 8 24 0 100 5 10
    240613155913_2b5n ATTGGAAGTTCACTTCCTCTGAGCAAAATTAG(+AGGACA)GATCCTGATCCCTCCTCCCTTGGGCCCCATG NGG 10 8 17 8 24 0 100 5 10
    240613155912_83iv AGGCAGATCCTGTGGTTCTGGAAGATACTCTG(+AGGACA)GATCCAGAGTCCTTGGTAGAGAAGCCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613155911_y2ae TCACGCTGGAGGCTTCTCTACCAAGGACTCTG(+AGGACA)GATCCAGAGTATCTTCCAGAACCACAGGATC NGG 10 8 17 8 24 0 100 5 10
    240613155909_q96l TCTCTCTTTCCTTGTGTTTCCTTTACCTCCTG(+AGGACA)GTACCTGGAAGTCCCGCCCATACCTTCCACC NGG 10 8 17 8 24 0 100 5 10