Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155958_n34k AGGATGGCATTAGGAGCCAGGGCTGGCCATGG(+AGGACA)GTACCTTACTTTCCTCCCTGGGGTATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613155955_7wci GAAGGGAAGCTCTGCCCACCCTCAAGTCCCTG(+AGGACA)GATCCTGAGCCAGAGTCAATTCTATACTGTA NGG 10 8 17 8 24 0 100 5 10
    240613155954_hvrq GTGACTACAGTATAGAATTGACTCTGGCTCAG(+AGGACA)GATCCAGGGACTTGAGGGTGGGCAGAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613155952_don4 CAAGGCAGCACGCTCAGAGGACCTGGAGCAGG(+AGGACA)GATCCCGCAGTAAAGCAGCAGCAGATGTCTA NGG 10 8 17 8 24 0 100 5 10
    240613155951_dfd7 GTTTGTAGACATCTGCTGCTGCTTTACTGCGG(+AGGACA)GATCCCTGCTCCAGGTCCTCTGAGCGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613155949_5vb9 CTTCCTCCTTGAAAGCAGGGTTACCCTTAAAG(+AGGACA)GATCCCTGGAGCCCCTCCCATAAGAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240613155947_fder TCAACAGGGCCTCTTATGGGAGGGGCTCCAGG(+AGGACA)GATCCTTTAAGGGTAACCCTGCTTTCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613155946_wvfp GGTTGAGTTCTGGAGCAGTTGCAGGTAGCACG(+AGGACA)GTACCCCGGAAATGGGAAGTCTCTGGACAGT NGG 10 8 17 8 24 0 100 5 10
    240613155943_72ml TGAGGACTGTCCAGAGACTTCCCATTTCCGGG(+AGGACA)GTACCGTGCTACCTGCAACTGCTCCAGAACT NGG 10 8 17 8 24 0 100 5 10
    240613155942_7icy ATCTTCCCTCGGCTGATCCAGCAGCCTGGGTG(+AGGACA)GTACCGGTGTGCATTGCCGTATCCAGTCTTG NGG 10 8 17 8 24 0 100 5 10