Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155907_8ses TGCTGGGTGGAAGGTATGGGCGGGACTTCCAG(+AGGACA)GTACCAGGAGGTAAAGGAAACACAAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240613155906_0t2w GTAAACAGCAAGAGGCTTTATTGGGAACACGG(+AGGACA)GTACCCGGGCGACTCAGTCTATCGGAGGACT NGG 10 8 17 8 24 0 100 5 10
    240613155903_5pho GCGCCAGTCCTCCGATAGACTGAGTCGCCCGG(+AGGACA)GTACCCGTGTTCCCAATAAAGCCTCTTGCTG NGG 10 8 17 8 24 0 100 5 10
    240613155902_lk0c TTCTCCATGGTGTGAGTGGCTCGTCTTGTAGG(+AGGACA)GATCCTTCATGTGTCTCACCAACTGGCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613155900_s1or GAAAAGGCAGCCAGTTGGTGAGACACATGAAG(+AGGACA)GATCCCTACAAGACGAGCCACTCACACCATG NGG 10 8 17 8 24 0 100 5 10
    240613155857_qgl5 ATTGGCTGGATGGGATTGCCCTATTCCCCAGG(+AGGACA)GATCCTCCTATTTCTGCTCCCCATTGCTAGG NGG 10 8 17 8 24 0 100 5 10
    240613155856_ddfx ATAATCCTAGCAATGGGGAGCAGAAATAGGAG(+AGGACA)GATCCCTGGGGAATAGGGCAATCCCATCCAG NGG 10 8 17 8 24 0 100 5 10
    240613155854_xtgb AAAAGACCTCAGGCACCTGAGCACTTTGACAG(+AGGACA)GTACCAGGTAGAGTCCATCCTTGCATCAGTG NGG 10 8 17 8 24 0 100 5 10
    240613155852_4yrm AGGAACACTGATGCAAGGATGGACTCTACCTG(+AGGACA)GTACCTGTCAAAGTGCTCAGGTGCCTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240613155850_kae6 GCAGGTCCCACGGTGTTTGGAGATGGCTTGGG(+AGGACA)GATCCGTGAGGATGGCATGACCAGCTAGCCC NGG 10 8 17 8 24 0 100 5 10