Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155758_9ves CCAGGTACTCAGATGTCTGGAATAGGTAACAG(+AGGACA)GTACCCCGGAACTCACAGGCCTGTCCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613155757_yn4h TACCCTGAGCCAGGCATTTTAACACTACAGTG(+AGGACA)GTACCCACACAAGGCACATGGGATGATTAGC NGG 10 8 17 8 24 0 100 5 10
    240613155755_mtg8 AAGGTGCTAATCATCCCATGTGCCTTGTGTGG(+AGGACA)GTACCACTGTAGTGTTAAAATGCCTGGCTCA NGG 10 8 17 8 24 0 100 5 10
    240613155754_oruz AGCTGCTGTGAAGAGGGCAAGGGTTAAAAATG(+AGGACA)GATCCACTGCTGCTTTCCTTTTTCCTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613155753_7xuy AATAAAAAGAAGGAAAAAGGAAAGCAGCAGTG(+AGGACA)GATCCATTTTTAACCCTTGCCCTCTTCACAG NGG 10 8 17 8 24 0 100 5 10
    240613155751_rqlw CTAGACATCATTCATAGAAACAATAAAGAATG(+AGGACA)GTACCAATGTAGAGGAGCAAAGCTGCTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613155750_wtx8 AGGAAACCCAGCAGCTTTGCTCCTCTACATTG(+AGGACA)GTACCATTCTTTATTGTTTCTATGAATGATG NGG 10 8 17 8 24 0 100 5 10
    240613155749_akfp AAACATTGAATCTCAGGGATGGTCCACAGCTG(+AGGACA)GATCCAAACTTAACGAGCCCTGTTTGAATAA NGG 10 8 17 8 24 0 100 5 10
    240613155748_k2k8 CTTCATTATTCAAACAGGGCTCGTTAAGTTTG(+AGGACA)GATCCAGCTGTGGACCATCCCTGAGATTCAA NGG 10 8 17 8 24 0 100 5 10
    240613155745_b83f CAAATCACTCCTGTGCATAGCACTTTCCCTGG(+AGGACA)GATCCACAATTCAGCCCTGGGTCCCCACCCC NGG 10 8 17 8 24 0 100 5 10