Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155744_xvlf GGTAAGGGGTGGGGACCCAGGGCTGAATTGTG(+AGGACA)GATCCCAGGGAAAGTGCTATGCACAGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240613155742_6nh0 ATTATCCAACTCCCACCCCAGGATCTCCACTG(+AGGACA)GTACCCAGATGCCCTTAGACCCTTTCATCTG NGG 10 8 17 8 24 0 100 5 10
    240613155740_iyuy GCTTCCAGATGAAAGGGTCTAAGGGCATCTGG(+AGGACA)GTACCAGTGGAGATCCTGGGGTGGGAGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613155739_uz2a ACCTCAAGGCTTGGGTGACACACACAAATGTG(+AGGACA)GTACCTCAGCCAGGGCCAGGACCTGCTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613155738_ziir TGGACCCCTAGCAGGTCCTGGCCCTGGCTGAG(+AGGACA)GTACCACATTTGTGTGTGTCACCCAAGCCTT NGG 10 8 17 8 24 0 100 5 10
    240613155736_r8lj TTCTACAGGCTTAGGGGCCTTTCCAGGGATGG(+AGGACA)GTACCACCAAGGACTAACCACTTTACTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613155735_yhxd GGGGAACAAAGTAAAGTGGTTAGTCCTTGGTG(+AGGACA)GTACCCATCCCTGGAAAGGCCCCTAAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613155734_sief TCTAATAGGTCATCCTGTGGCTTCCAGAGTAG(+AGGACA)GTACCGCCAGCCCAGCTCAAGTTCTCTGCTT NGG 10 8 17 8 24 0 100 5 10
    240613155732_7x81 CCGTTAAGCAGAGAACTTGAGCTGGGCTGGCG(+AGGACA)GTACCTACTCTGGAAGCCACAGGATGACCTA NGG 10 8 17 8 24 0 100 5 10
    240613155731_lkg7 TTCTTTTGTTTCTTATAAGACGAATATTGAGG(+AGGACA)GATCCTTGTATTTCTTTCTTTTGTATTTTTT NGG 10 8 17 8 24 0 100 5 10