Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613155815_bqgv CCCGGCTGAAGGCACAAACGCTGTGGTTAGGG(+AGGACA)GTACCAAGGCAAGGCATGGATTCATTCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613155813_hnpu TGCTTTGTTTGAATAAACAGTATATCTTTGTG(+AGGACA)GATCCTACTTTCTTTGACTGTAAAGGATTCT NGG 10 8 17 8 24 0 100 5 10
    240613155812_im0k TTACAAGAATCCTTTACAGTCAAAGAAAGTAG(+AGGACA)GATCCACAAAGATATACTGTTTATTCAAACA NGG 10 8 17 8 24 0 100 5 10
    240613155811_kkwz CAAGAACAACTTAGAAGGTGGGCAGAAGGCAG(+AGGACA)GTACCTCAGCTCCTTCAGCTTCTTTTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613155810_siu2 AGATTGAGGAAAAAGAAGCTGAAGGAGCTGAG(+AGGACA)GTACCTGCCTTCTGCCCACCTTCTAAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613155808_mz1d CCGAGCATAATCTCTAGGAAGGTTTTCTTTAG(+AGGACA)GATCCCAAGGAGATGTCCGGAAACTCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240613155806_tnpg GAGGCCTCAAGAGTTTCCGGACATCTCCTTGG(+AGGACA)GATCCTAAAGAAAACCTTCCTAGAGATTATG NGG 10 8 17 8 24 0 100 5 10
    240613155804_m2s0 TGCAGCTGGGGGTGGCAAAGTGCAGAGCCCAG(+AGGACA)GTACCAGGCCCCTGAGCTCCAGCAGGGAGAG NGG 10 8 17 8 24 0 100 5 10
    240613155802_bswj TCTGCCTCTCCCTGCTGGAGCTCAGGGGCCTG(+AGGACA)GTACCTGGGCTCTGCACTTTGCCACCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613155759_9fnf CTTCCTCCAAGGACAGGCCTGTGAGTTCCGGG(+AGGACA)GTACCTGTTACCTATTCCAGACATCTGAGTA NGG 10 8 17 8 24 0 100 5 10